X-irradiation-induced disorganization of cytoskeletal filaments and cell contacts in HT29 cells

Z. Somosy, M. Sass, G. Bognar, J. Kovács, G. J. Koteles, M. Kasper, P. B. Bell, L. S. Yasui, M. Malecki

Research output: Article

15 Citations (Scopus)

Abstract

Organization of cytoskeleton and cell contacts were studied by immunochemistry and electron microscopy in confluent HT29 cultured cells following exposure to 0.5 and 1.0 Gy doses of X-ray. Microtubules were resistant to irradiation, whereas, the actin and intermediate filaments disrupted rapidly following the treatment and their components appeared as clumps of actin and cytokeratin aggregates in the cytoplasm as demonstrated by immunochemistry. Loss of cell contacts and decrease in the number of desmosomes was also characteristic of irradiated cells. Electron microscopy revealed intact desmosomes in control cells and abnormal desmosomes in the irradiated samples characterized by the absence of tonofilaments. The perinuclear filament network and cortical filaments were well detectable by electron microscopy. Under the effect of irradiation, the perinuclear filaments almost disappeared and, at the same time, small bundles of filaments were formed irregularly in the cytoplasm associated with amorphous material.

Original languageEnglish
Pages (from-to)763-772
Number of pages10
JournalScanning Microscopy
Volume9
Issue number3
Publication statusPublished - 1995

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Electron microscopy
filaments
Irradiation
irradiation
cells
electron microscopy
Cells
cytoplasm
Dosimetry
amorphous materials
X rays
clumps
cultured cells
bundles
microscopy
dosage
Immunochemistry
x rays

ASJC Scopus subject areas

  • Instrumentation

Cite this

X-irradiation-induced disorganization of cytoskeletal filaments and cell contacts in HT29 cells. / Somosy, Z.; Sass, M.; Bognar, G.; Kovács, J.; Koteles, G. J.; Kasper, M.; Bell, P. B.; Yasui, L. S.; Malecki, M.

In: Scanning Microscopy, Vol. 9, No. 3, 1995, p. 763-772.

Research output: Article

Somosy, Z, Sass, M, Bognar, G, Kovács, J, Koteles, GJ, Kasper, M, Bell, PB, Yasui, LS & Malecki, M 1995, 'X-irradiation-induced disorganization of cytoskeletal filaments and cell contacts in HT29 cells', Scanning Microscopy, vol. 9, no. 3, pp. 763-772.
Somosy, Z. ; Sass, M. ; Bognar, G. ; Kovács, J. ; Koteles, G. J. ; Kasper, M. ; Bell, P. B. ; Yasui, L. S. ; Malecki, M. / X-irradiation-induced disorganization of cytoskeletal filaments and cell contacts in HT29 cells. In: Scanning Microscopy. 1995 ; Vol. 9, No. 3. pp. 763-772.
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AU - Somosy, Z.

AU - Sass, M.

AU - Bognar, G.

AU - Kovács, J.

AU - Koteles, G. J.

AU - Kasper, M.

AU - Bell, P. B.

AU - Yasui, L. S.

AU - Malecki, M.

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AB - Organization of cytoskeleton and cell contacts were studied by immunochemistry and electron microscopy in confluent HT29 cultured cells following exposure to 0.5 and 1.0 Gy doses of X-ray. Microtubules were resistant to irradiation, whereas, the actin and intermediate filaments disrupted rapidly following the treatment and their components appeared as clumps of actin and cytokeratin aggregates in the cytoplasm as demonstrated by immunochemistry. Loss of cell contacts and decrease in the number of desmosomes was also characteristic of irradiated cells. Electron microscopy revealed intact desmosomes in control cells and abnormal desmosomes in the irradiated samples characterized by the absence of tonofilaments. The perinuclear filament network and cortical filaments were well detectable by electron microscopy. Under the effect of irradiation, the perinuclear filaments almost disappeared and, at the same time, small bundles of filaments were formed irregularly in the cytoplasm associated with amorphous material.

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KW - cytokeratin

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KW - electron microscopy

KW - HT-29 cells

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KW - int ermediate filaments

KW - microtubules

KW - vimentin

KW - vinculin

KW - X- irradiation

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