Without Binding ATP, Human Rad51 Does Not Form Helical Filaments on ssDNA

Gusztáv Schay, Bálint Borka, Linda Kernya, Éva Bulyáki, József Kardos, Melinda Fekete, Judit Fidy

Research output: Article

3 Citations (Scopus)

Abstract

Construction of the presynaptic filament (PSF) of proper helical structure by Rad51 recombinases is a prerequisite of the progress of homologous recombination repair. We studied the contribution of ATP-binding to this structure of wt human Rad51 (hRad51). We exploited the protein-dissociation effect of high hydrostatic pressure to determine the free energy of dissociation of the protomer interfaces in hRad51 oligomer states and used electron microscopy to obtain topological parameters. Without cofactors ATP and Ca2+ and template DNA, hRad51 did not exist in monomer form, but it formed rodlike long filaments without helical order. ΔGdiss indicated a strong inherent tendency of aggregation. Binding solely ssDNA left the filament unstructured with slightly increased ΔGdiss. Adding only ATP and Ca2+ to the buffer disintegrated the self-associated rods into rings and short helices of further increased ΔGdiss. Rad51 binding to ssDNA only with ATP and Ca bound could lead to ordered helical filament formation of proper pitch size with interface contacts of Kd ∼ 2 × 10-11 M, indicating a structure of outstanding stability. ATP/Ca binding increased the ΔGdiss of protomer contacts in the filament by 16 kJ/mol. The results emphasize that ATP-binding in the PSF of hRad51 has an essential, yet purely structural, role.

Original languageEnglish
Pages (from-to)2165-2178
Number of pages14
JournalJournal of Physical Chemistry B
Volume120
Issue number9
DOIs
Publication statusPublished - márc. 17 2016

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ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Surfaces, Coatings and Films

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