Wide-line NMR and DSC studies on intrinsically disordered p53 transactivation domain and its helically pre-structured segment

Peter Tompa, Kyou Hoon Han, M. Bokor, Pawel Kamasa, Ágnes Tantos, Beáta Fritz, Do Hyoung Kim, Chewook Lee, Tamás Verebélyi, K. Tompa

Research output: Article

3 Citations (Scopus)

Abstract

Wide-line 1H NMR intensity and differential scanning calorimetry measurements were carried out on the intrinsically disordered 73-residue full transactivation domain (TAD) of the p53 tumor suppressor protein and two peptides: one a wild type p53 TAD peptide with a helix pre-structuring property, and a mutant peptide with a disabled helix-forming propensity. Measurements were carried out in order to characterize their water and ion binding characteristics. By quantifying the number of hydrate water molecules, we provide a microscopic description for the interactions of water with a wild-type p53 TAD and two p53 TAD peptides. The results provide direct evidence that intrinsically disordered proteins (IDPs) and a less structured peptide not only have a higher hydration capacity than globular proteins, but are also able to bind a larger amount of charged solute ions.

Original languageEnglish
Pages (from-to)497-501
Number of pages5
JournalBMB Reports
Volume49
Issue number9
DOIs
Publication statusPublished - 2016

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Transcriptional Activation
Nuclear magnetic resonance
Peptides
Water
Intrinsically Disordered Proteins
Ions
Tumor Suppressor Protein p53
Differential Scanning Calorimetry
Hydrates
Hydration
Differential scanning calorimetry
Molecules
Proteins
Protein Domains

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Wide-line NMR and DSC studies on intrinsically disordered p53 transactivation domain and its helically pre-structured segment. / Tompa, Peter; Han, Kyou Hoon; Bokor, M.; Kamasa, Pawel; Tantos, Ágnes; Fritz, Beáta; Kim, Do Hyoung; Lee, Chewook; Verebélyi, Tamás; Tompa, K.

In: BMB Reports, Vol. 49, No. 9, 2016, p. 497-501.

Research output: Article

Tompa, Peter ; Han, Kyou Hoon ; Bokor, M. ; Kamasa, Pawel ; Tantos, Ágnes ; Fritz, Beáta ; Kim, Do Hyoung ; Lee, Chewook ; Verebélyi, Tamás ; Tompa, K. / Wide-line NMR and DSC studies on intrinsically disordered p53 transactivation domain and its helically pre-structured segment. In: BMB Reports. 2016 ; Vol. 49, No. 9. pp. 497-501.
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AU - Kamasa, Pawel

AU - Tantos, Ágnes

AU - Fritz, Beáta

AU - Kim, Do Hyoung

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