Whole genome transcriptional profiling identifies novel differentiation regulated genes in keratinocytes.

György Paragh, Peter Ugocsai, Thomas Vogt, Petra Schling, Alexander E. Kel, Victoria Tarabin, Gerhard Liebisch, Evelyn Orsó, Loránt Markó, Attila Balogh, Tamás Köbling, E. Remenyik, N. Wikonkál, J. Mandl, Mike Farwick, Gerd Schmitz

Research output: Article

7 Citations (Scopus)

Abstract

Keratinocyte differentiation plays a pivotal role in the epidermal barrier. Single keratinocyte differentiation genes have already been studied, but many important constituents of this process may have been missed so far. Gene expression profiling by microarray was carried out in cultured normal human epidermal keratinocytes undergoing confluence-induced differentiation to find novel differentiation genes. Candidate gene lists were established and genes of potential dermatological interest were validated by quantitative reverse transcription polymerase chain reaction and immunohistochemical analysis. Some of these points lead to the identification of counter-regulation of heme oxygenase and biliverdin reductase as well as glutaredoxin and glutathione reductase indicative of potential novel redox signaling in differentiating human keratinocytes. Others indicate a strong concert down-regulation of interleukin-1 signaling at previously unidentified levels during keratinocyte differentiation. We believe that identified genes contribute to a more comprehensive understanding of the complicated epidermal differentiation process and lead to better understanding of dermatological diseases.

Original languageEnglish
Pages (from-to)297-301
Number of pages5
JournalExperimental Dermatology
Volume19
Issue number3
DOIs
Publication statusPublished - márc. 2010

Fingerprint

Keratinocytes
Genes
Genome
biliverdin reductase
Glutaredoxins
Heme Oxygenase (Decyclizing)
Glutathione Reductase
Polymerase chain reaction
Gene Expression Profiling
Transcription
Microarrays
Interleukin-1
Gene expression
Reverse Transcription
Oxidation-Reduction
Down-Regulation
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Dermatology
  • Molecular Biology
  • Biochemistry

Cite this

Whole genome transcriptional profiling identifies novel differentiation regulated genes in keratinocytes. / Paragh, György; Ugocsai, Peter; Vogt, Thomas; Schling, Petra; Kel, Alexander E.; Tarabin, Victoria; Liebisch, Gerhard; Orsó, Evelyn; Markó, Loránt; Balogh, Attila; Köbling, Tamás; Remenyik, E.; Wikonkál, N.; Mandl, J.; Farwick, Mike; Schmitz, Gerd.

In: Experimental Dermatology, Vol. 19, No. 3, 03.2010, p. 297-301.

Research output: Article

Paragh, G, Ugocsai, P, Vogt, T, Schling, P, Kel, AE, Tarabin, V, Liebisch, G, Orsó, E, Markó, L, Balogh, A, Köbling, T, Remenyik, E, Wikonkál, N, Mandl, J, Farwick, M & Schmitz, G 2010, 'Whole genome transcriptional profiling identifies novel differentiation regulated genes in keratinocytes.', Experimental Dermatology, vol. 19, no. 3, pp. 297-301. https://doi.org/10.1111/j.1600-0625.2009.00920.x
Paragh, György ; Ugocsai, Peter ; Vogt, Thomas ; Schling, Petra ; Kel, Alexander E. ; Tarabin, Victoria ; Liebisch, Gerhard ; Orsó, Evelyn ; Markó, Loránt ; Balogh, Attila ; Köbling, Tamás ; Remenyik, E. ; Wikonkál, N. ; Mandl, J. ; Farwick, Mike ; Schmitz, Gerd. / Whole genome transcriptional profiling identifies novel differentiation regulated genes in keratinocytes. In: Experimental Dermatology. 2010 ; Vol. 19, No. 3. pp. 297-301.
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