Vitamin E, LDL, and endothelium: Brief oral vitamin supplementation prevents oxidized LDL-mediated vascular injury in vitro

John D. Belcher, J. Balla, G. Balla, David R. Jacobs, Myron Gross, Harry S. Jacob, Gregory M. Vercellotti

Research output: Article

89 Citations (Scopus)

Abstract

In previously reported in vitro studies, we found that heme, a physiologically widespread hydrophobic iron compound, can rapidly generate oxidized low-density lipoprotein (LDL), which then becomes cytotoxic to cultured vascular endothelial cells; both LDL oxidation and endothelial cytotoxicity were inhibited by incubation with exogenous α-tocopherol (vitamin E) or ascorbic acid (vitamin C). Seeking relevance to in vivo conditions, we performed a study in which 10 human volunteers were given daily antioxidant supplements of 800 IU of DL-α-tocopherol acetate alone or in combination with 1000 mg of ascorbic acid for 2 weeks. LDL resistance to heme oxidation ex vivo, as measured by the lag time for conjugated-diene formation, increased by as much as threefold from a mean±SD of 58±11 to 104±18 minutes (P2O2 or with copper was completely prevented (cytotoxicity before supplementation was 42±12%, decreasing after supplementation to 3±2%, P

Original languageEnglish
Pages (from-to)1779-1789
Number of pages11
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume13
Issue number12
Publication statusPublished - 1993

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Vascular System Injuries
Vitamin E
LDL Lipoproteins
Vitamins
Ascorbic Acid
Endothelium
Iron Compounds
Tocopherols
alpha-Tocopherol
Heme
Copper
Volunteers
Endothelial Cells
Antioxidants
oxidized low density lipoprotein
In Vitro Techniques

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Vitamin E, LDL, and endothelium : Brief oral vitamin supplementation prevents oxidized LDL-mediated vascular injury in vitro. / Belcher, John D.; Balla, J.; Balla, G.; Jacobs, David R.; Gross, Myron; Jacob, Harry S.; Vercellotti, Gregory M.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 13, No. 12, 1993, p. 1779-1789.

Research output: Article

Belcher, John D. ; Balla, J. ; Balla, G. ; Jacobs, David R. ; Gross, Myron ; Jacob, Harry S. ; Vercellotti, Gregory M. / Vitamin E, LDL, and endothelium : Brief oral vitamin supplementation prevents oxidized LDL-mediated vascular injury in vitro. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 1993 ; Vol. 13, No. 12. pp. 1779-1789.
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AU - Jacob, Harry S.

AU - Vercellotti, Gregory M.

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