The effect of four sperm-egg ratios on the fertilizing capacity of cryopreserved sperm of the genetically distinct Adriatic grayling (Thymallus thymallus) was investigated. Sperm was diluted at a ratio of 1:1 in an extender composed of 200mM glucose, 40mM KCl, 30mM Tris (pH8.0) and 10% methanol and frozen in the vapor of liquid nitrogen. Fertilization tests were conducted with sperm-egg ratios ranging from 103 to 5×104 spermatozoa to one egg. Percentages of eyed eggs (60±7%) and hatched larvae (53±7%) were not significantly different from the control (68±3% eyed eggs and 55±3% hatched larvae) with the sperm-egg ratio of 5×104 spermatozoa to one egg. Cryopreserved sperm retained its fertilizing capacity even with the lowest tested sperm-egg ratio.
ASJC Scopus subject areas
- Aquatic Science