Uridine modulates neuronal activity and inhibits spike-wave discharges of absence epileptic Long Evans and Wistar Albino Glaxo/Rijswijk rats

Z. Kovács, Andrea Slézia, Zsolt Kristóf Bali, Péter Kovács, A. Dobolyi, Tamás Szikra, I. Hernádi, G. Juhász

Research output: Article

12 Citations (Scopus)

Abstract

Pharmacological and functional data suggest the existence of uridine (Urd) receptors in the central nervous system (CNS). In the present study, simultaneous extracellular single unit recording and microiontophoretic injection of the pyrimidine nucleoside Urd was used to provide evidence for the presence of Urd-sensitive neurons in the thalamus and the cerebral cortex of Long Evans rats. Twenty-two neurons in the thalamus (24% of recorded neurons) and 17 neurons in the cortex (55%) responded to the direct iontophoresis of Urd. The majority of Urd-sensitive neurons in the thalamus and cortex (82% and 59%, respectively) increased their firing rate in response to Urd. In contrary, adenosine (Ado) and uridine 5'-triphosphate (UTP) decreased the firing rate of all responding neurons in the thalamus, and the majority of responding neurons in the cortex (83% and 87%, respectively). Functional relevance of Urd-sensitive neurons was investigated in spontaneously epileptic freely moving Long Evans and Wistar Albino Glaxo/Rijswijk (WAG/Rij) rats. Intraperitoneal (i.p.) injection of 500. mg/kg Urd decreased epileptic activity (210-270. min after injection) in both rat strains. Intraperitoneal administration of 1000. mg/kg Urd decreased the number of spike-wave discharges (SWDs) between 150-270. min and 90-270. min in Long Evans and WAG/Rij rats, respectively. The effect of Urd was long-lasting in both rat strains as the higher dose significantly decreased the number of SWDs even 24. h after Urd injection. The present results suggest that Urd-sensitive neurons in the thalamus and the cerebral cortex may play a role in the antiepileptic action of Urd possibly via modulation of thalamocortical neuronal circuits.

Original languageEnglish
Pages (from-to)16-23
Number of pages8
JournalBrain Research Bulletin
Volume97
DOIs
Publication statusPublished - aug. 2013

Fingerprint

Uridine
Neurons
Thalamus
Cerebral Cortex
Injections
Pyrimidine Nucleosides
Iontophoresis
Long Evans Rats
Intraperitoneal Injections
Anticonvulsants
Central Nervous System

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "Uridine modulates neuronal activity and inhibits spike-wave discharges of absence epileptic Long Evans and Wistar Albino Glaxo/Rijswijk rats",
abstract = "Pharmacological and functional data suggest the existence of uridine (Urd) receptors in the central nervous system (CNS). In the present study, simultaneous extracellular single unit recording and microiontophoretic injection of the pyrimidine nucleoside Urd was used to provide evidence for the presence of Urd-sensitive neurons in the thalamus and the cerebral cortex of Long Evans rats. Twenty-two neurons in the thalamus (24{\%} of recorded neurons) and 17 neurons in the cortex (55{\%}) responded to the direct iontophoresis of Urd. The majority of Urd-sensitive neurons in the thalamus and cortex (82{\%} and 59{\%}, respectively) increased their firing rate in response to Urd. In contrary, adenosine (Ado) and uridine 5'-triphosphate (UTP) decreased the firing rate of all responding neurons in the thalamus, and the majority of responding neurons in the cortex (83{\%} and 87{\%}, respectively). Functional relevance of Urd-sensitive neurons was investigated in spontaneously epileptic freely moving Long Evans and Wistar Albino Glaxo/Rijswijk (WAG/Rij) rats. Intraperitoneal (i.p.) injection of 500. mg/kg Urd decreased epileptic activity (210-270. min after injection) in both rat strains. Intraperitoneal administration of 1000. mg/kg Urd decreased the number of spike-wave discharges (SWDs) between 150-270. min and 90-270. min in Long Evans and WAG/Rij rats, respectively. The effect of Urd was long-lasting in both rat strains as the higher dose significantly decreased the number of SWDs even 24. h after Urd injection. The present results suggest that Urd-sensitive neurons in the thalamus and the cerebral cortex may play a role in the antiepileptic action of Urd possibly via modulation of thalamocortical neuronal circuits.",
keywords = "Absence epileptic rats, EEG, Extracellular neuronal activity, Multibarrel microiontophoresis, Pyrimidine nucleoside, Spike-wave discharges",
author = "Z. Kov{\'a}cs and Andrea Sl{\'e}zia and Bali, {Zsolt Krist{\'o}f} and P{\'e}ter Kov{\'a}cs and A. Dobolyi and Tam{\'a}s Szikra and I. Hern{\'a}di and G. Juh{\'a}sz",
year = "2013",
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language = "English",
volume = "97",
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T1 - Uridine modulates neuronal activity and inhibits spike-wave discharges of absence epileptic Long Evans and Wistar Albino Glaxo/Rijswijk rats

AU - Kovács, Z.

AU - Slézia, Andrea

AU - Bali, Zsolt Kristóf

AU - Kovács, Péter

AU - Dobolyi, A.

AU - Szikra, Tamás

AU - Hernádi, I.

AU - Juhász, G.

PY - 2013/8

Y1 - 2013/8

N2 - Pharmacological and functional data suggest the existence of uridine (Urd) receptors in the central nervous system (CNS). In the present study, simultaneous extracellular single unit recording and microiontophoretic injection of the pyrimidine nucleoside Urd was used to provide evidence for the presence of Urd-sensitive neurons in the thalamus and the cerebral cortex of Long Evans rats. Twenty-two neurons in the thalamus (24% of recorded neurons) and 17 neurons in the cortex (55%) responded to the direct iontophoresis of Urd. The majority of Urd-sensitive neurons in the thalamus and cortex (82% and 59%, respectively) increased their firing rate in response to Urd. In contrary, adenosine (Ado) and uridine 5'-triphosphate (UTP) decreased the firing rate of all responding neurons in the thalamus, and the majority of responding neurons in the cortex (83% and 87%, respectively). Functional relevance of Urd-sensitive neurons was investigated in spontaneously epileptic freely moving Long Evans and Wistar Albino Glaxo/Rijswijk (WAG/Rij) rats. Intraperitoneal (i.p.) injection of 500. mg/kg Urd decreased epileptic activity (210-270. min after injection) in both rat strains. Intraperitoneal administration of 1000. mg/kg Urd decreased the number of spike-wave discharges (SWDs) between 150-270. min and 90-270. min in Long Evans and WAG/Rij rats, respectively. The effect of Urd was long-lasting in both rat strains as the higher dose significantly decreased the number of SWDs even 24. h after Urd injection. The present results suggest that Urd-sensitive neurons in the thalamus and the cerebral cortex may play a role in the antiepileptic action of Urd possibly via modulation of thalamocortical neuronal circuits.

AB - Pharmacological and functional data suggest the existence of uridine (Urd) receptors in the central nervous system (CNS). In the present study, simultaneous extracellular single unit recording and microiontophoretic injection of the pyrimidine nucleoside Urd was used to provide evidence for the presence of Urd-sensitive neurons in the thalamus and the cerebral cortex of Long Evans rats. Twenty-two neurons in the thalamus (24% of recorded neurons) and 17 neurons in the cortex (55%) responded to the direct iontophoresis of Urd. The majority of Urd-sensitive neurons in the thalamus and cortex (82% and 59%, respectively) increased their firing rate in response to Urd. In contrary, adenosine (Ado) and uridine 5'-triphosphate (UTP) decreased the firing rate of all responding neurons in the thalamus, and the majority of responding neurons in the cortex (83% and 87%, respectively). Functional relevance of Urd-sensitive neurons was investigated in spontaneously epileptic freely moving Long Evans and Wistar Albino Glaxo/Rijswijk (WAG/Rij) rats. Intraperitoneal (i.p.) injection of 500. mg/kg Urd decreased epileptic activity (210-270. min after injection) in both rat strains. Intraperitoneal administration of 1000. mg/kg Urd decreased the number of spike-wave discharges (SWDs) between 150-270. min and 90-270. min in Long Evans and WAG/Rij rats, respectively. The effect of Urd was long-lasting in both rat strains as the higher dose significantly decreased the number of SWDs even 24. h after Urd injection. The present results suggest that Urd-sensitive neurons in the thalamus and the cerebral cortex may play a role in the antiepileptic action of Urd possibly via modulation of thalamocortical neuronal circuits.

KW - Absence epileptic rats

KW - EEG

KW - Extracellular neuronal activity

KW - Multibarrel microiontophoresis

KW - Pyrimidine nucleoside

KW - Spike-wave discharges

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