Two-dimensional correlation analysis of the reproducibility of high-performance liquid chromatography columns

József Simon, Attila Felinger

Research output: Article

11 Citations (Scopus)


Two-dimensional (2D) correlation analysis is a well-established tool in spectroscopy. Despite its versatility in various measurement systems, 2D correlation has not yet become popular in separation science. 2D correlation is seldom used in chromatography; only a few a studies can be found on this topic and most of those publications report about gel chromatography. In the present study, 2D correlation analysis is applied to chromatograms. In this study, a simple method is built for studying the similarities and dissimilarities between a number of chromatograms. We present the applicability of the method by two examples, where the repeatability and reproducibility of the analytical and nonlinear measurements in HPLC are evaluated and demonstrated. In order to validate the results of 2D correlation analysis, they are compared to principal component analysis (PCA). We confirm the equivalence in the interpretation of the results obtained with the two methods of calculation. The results confirm that 2D correlation can be a successful chemometric tool in chromatography.

Original languageEnglish
Pages (from-to)115-123
Number of pages9
JournalJournal of Chromatography A
Publication statusPublished - márc. 6 2015


ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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