Twisting biological objects by optical tweezers

P. Ormos, Herbert Van Amerongen, S. Bottka, P. Galajda, G. Garab, Huba Kirei, László Oroszi

Research output: Conference contribution

1 Citation (Scopus)

Abstract

We describe a novel method by which it is possible to apply and measure torque directly on particles grabbed in optical tweezers. It can be used to orient particles of micron size or even on single molecules, biopolymers by the use of test particles. The procedure is based on the observation that flat objects are oriented in an optical trap formed by linearly polarized light. The orienting torque originates from the anisotropic scattering of polarized light by the trapped particle. The phenomenon is characterized in detail, the physics is analysed. A tool is developed that exploits this effect to manuipulate biological macromolecules. Microscopic particles are produced by photopolymerisation that exploit this orientation effect and by which the torque is applied upon the biological object. In our system the applied torque can be turned on and off, it is controlled independently of the grabbing force of the tweezers during the manipulation process. The capabilities of the method are demonstrated. The method has great promise for application on DNA, DNA-protein complexes, actin filaments and other biopolymers.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsK. Dholakia, G.C. Spalding
Pages207-214
Number of pages8
Volume5514
DOIs
Publication statusPublished - 2004
EventOptical Trapping and Optical Micromanipulation - Denver, CO, United States
Duration: aug. 2 2004aug. 6 2004

Other

OtherOptical Trapping and Optical Micromanipulation
CountryUnited States
CityDenver, CO
Period8/2/048/6/04

Fingerprint

Optical tweezers
twisting
torque
Torque
biopolymers
Biopolymers
Light polarization
polarized light
DNA
deoxyribonucleic acid
Photopolymerization
trapped particles
Macromolecules
macromolecules
manipulators
filaments
Physics
traps
Scattering
proteins

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Ormos, P., Van Amerongen, H., Bottka, S., Galajda, P., Garab, G., Kirei, H., & Oroszi, L. (2004). Twisting biological objects by optical tweezers. In K. Dholakia, & G. C. Spalding (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 5514, pp. 207-214). [26] https://doi.org/10.1117/12.558685

Twisting biological objects by optical tweezers. / Ormos, P.; Van Amerongen, Herbert; Bottka, S.; Galajda, P.; Garab, G.; Kirei, Huba; Oroszi, László.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / K. Dholakia; G.C. Spalding. Vol. 5514 2004. p. 207-214 26.

Research output: Conference contribution

Ormos, P, Van Amerongen, H, Bottka, S, Galajda, P, Garab, G, Kirei, H & Oroszi, L 2004, Twisting biological objects by optical tweezers. in K Dholakia & GC Spalding (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 5514, 26, pp. 207-214, Optical Trapping and Optical Micromanipulation, Denver, CO, United States, 8/2/04. https://doi.org/10.1117/12.558685
Ormos P, Van Amerongen H, Bottka S, Galajda P, Garab G, Kirei H et al. Twisting biological objects by optical tweezers. In Dholakia K, Spalding GC, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 5514. 2004. p. 207-214. 26 https://doi.org/10.1117/12.558685
Ormos, P. ; Van Amerongen, Herbert ; Bottka, S. ; Galajda, P. ; Garab, G. ; Kirei, Huba ; Oroszi, László. / Twisting biological objects by optical tweezers. Proceedings of SPIE - The International Society for Optical Engineering. editor / K. Dholakia ; G.C. Spalding. Vol. 5514 2004. pp. 207-214
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