Transformation-specific matrix metalloproteinases (MMP)-7 and MMP-13 are expressed by tumour cells in epidermolysis bullosa-associated squamous cell carcinomas

A. K. Kivisaari, M. Kallajoki, T. Mirtti, J. A. McGrath, J. W. Bauer, F. Weber, R. Königová, D. Sawamura, K. C. Sato-Matsumura, H. Shimizu, M. Csikós, K. Sinemus, W. Beckert, V. M. Kähäri

Research output: Article

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Abstract

Background: Patients with recessive dystrophic epidermolysis bullosa (RDEB) have an increased risk of developing rapidly progressive and metastatic cutaneous squamous cell carcinomas (SCC). It is unclear why these SCC behave more aggressively than sporadic SCC. Matrix metalloproteinases (MMP) are a family of endopeptidases that contribute to growth, invasion and metastasis of SCC. The role of MMP in RDEB-associated SCC is not known. Objectives: To investigate the expression of MMP-7, MMP-13 and MMP-9 in RDEB-associated SCC in comparison with sporadic SCC and Bowen's disease. Methods: Immunohistochemical analysis of 25 RDEB-associated SCC, 61 sporadic SCC and 28 sporadic lesions of Bowen's disease was carried out using monoclonal antibodies for MMP-7, MMP-9, MMP-13 and E-cadherin and syndecan-1. Results: MMP-7 was detected in all RDEB-associated SCC, in tumour cells within the invasive edge, where E-cadherin and syndecan-1 were markedly diminished or absent. MMP-7 expression was also observed in 98% of sporadic SCC and in 68% of Bowen's diseases. MMP-7 staining was significantly stronger in RDEB-associated SCC than in sporadic SCC, and was most abundant in poorly differentiated tumours. MMP-13 was detected in tumour cells in 96% of RDEB-associated SCC and in all sporadic cutaneous SCC. MMP-9 was detected in the inflammatory cells in all SCC examined. Conclusions: These results identify MMP-7 and MMP-13 as tumour cell-specific markers for SCC progression and as potential therapeutic targets in RDEB-associated SCC. The pattern of immunolabelling suggests that MMP-7 may shed E-cadherin and syndecan-1 from the SCC cell surface.

Original languageEnglish
Pages (from-to)778-785
Number of pages8
JournalBritish Journal of Dermatology
Volume158
Issue number4
DOIs
Publication statusPublished - ápr. 2008

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Matrix Metalloproteinase 7
Matrix Metalloproteinase 13
Epidermolysis Bullosa
Squamous Cell Carcinoma
Epidermolysis Bullosa Dystrophica
Neoplasms
Cadherins
Syndecan-1
Bowen's Disease
Matrix Metalloproteinase 9
Matrix Metalloproteinases

ASJC Scopus subject areas

  • Dermatology

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Transformation-specific matrix metalloproteinases (MMP)-7 and MMP-13 are expressed by tumour cells in epidermolysis bullosa-associated squamous cell carcinomas. / Kivisaari, A. K.; Kallajoki, M.; Mirtti, T.; McGrath, J. A.; Bauer, J. W.; Weber, F.; Königová, R.; Sawamura, D.; Sato-Matsumura, K. C.; Shimizu, H.; Csikós, M.; Sinemus, K.; Beckert, W.; Kähäri, V. M.

In: British Journal of Dermatology, Vol. 158, No. 4, 04.2008, p. 778-785.

Research output: Article

Kivisaari, AK, Kallajoki, M, Mirtti, T, McGrath, JA, Bauer, JW, Weber, F, Königová, R, Sawamura, D, Sato-Matsumura, KC, Shimizu, H, Csikós, M, Sinemus, K, Beckert, W & Kähäri, VM 2008, 'Transformation-specific matrix metalloproteinases (MMP)-7 and MMP-13 are expressed by tumour cells in epidermolysis bullosa-associated squamous cell carcinomas', British Journal of Dermatology, vol. 158, no. 4, pp. 778-785. https://doi.org/10.1111/j.1365-2133.2008.08466.x
Kivisaari, A. K. ; Kallajoki, M. ; Mirtti, T. ; McGrath, J. A. ; Bauer, J. W. ; Weber, F. ; Königová, R. ; Sawamura, D. ; Sato-Matsumura, K. C. ; Shimizu, H. ; Csikós, M. ; Sinemus, K. ; Beckert, W. ; Kähäri, V. M. / Transformation-specific matrix metalloproteinases (MMP)-7 and MMP-13 are expressed by tumour cells in epidermolysis bullosa-associated squamous cell carcinomas. In: British Journal of Dermatology. 2008 ; Vol. 158, No. 4. pp. 778-785.
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abstract = "Background: Patients with recessive dystrophic epidermolysis bullosa (RDEB) have an increased risk of developing rapidly progressive and metastatic cutaneous squamous cell carcinomas (SCC). It is unclear why these SCC behave more aggressively than sporadic SCC. Matrix metalloproteinases (MMP) are a family of endopeptidases that contribute to growth, invasion and metastasis of SCC. The role of MMP in RDEB-associated SCC is not known. Objectives: To investigate the expression of MMP-7, MMP-13 and MMP-9 in RDEB-associated SCC in comparison with sporadic SCC and Bowen's disease. Methods: Immunohistochemical analysis of 25 RDEB-associated SCC, 61 sporadic SCC and 28 sporadic lesions of Bowen's disease was carried out using monoclonal antibodies for MMP-7, MMP-9, MMP-13 and E-cadherin and syndecan-1. Results: MMP-7 was detected in all RDEB-associated SCC, in tumour cells within the invasive edge, where E-cadherin and syndecan-1 were markedly diminished or absent. MMP-7 expression was also observed in 98{\%} of sporadic SCC and in 68{\%} of Bowen's diseases. MMP-7 staining was significantly stronger in RDEB-associated SCC than in sporadic SCC, and was most abundant in poorly differentiated tumours. MMP-13 was detected in tumour cells in 96{\%} of RDEB-associated SCC and in all sporadic cutaneous SCC. MMP-9 was detected in the inflammatory cells in all SCC examined. Conclusions: These results identify MMP-7 and MMP-13 as tumour cell-specific markers for SCC progression and as potential therapeutic targets in RDEB-associated SCC. The pattern of immunolabelling suggests that MMP-7 may shed E-cadherin and syndecan-1 from the SCC cell surface.",
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T1 - Transformation-specific matrix metalloproteinases (MMP)-7 and MMP-13 are expressed by tumour cells in epidermolysis bullosa-associated squamous cell carcinomas

AU - Kivisaari, A. K.

AU - Kallajoki, M.

AU - Mirtti, T.

AU - McGrath, J. A.

AU - Bauer, J. W.

AU - Weber, F.

AU - Königová, R.

AU - Sawamura, D.

AU - Sato-Matsumura, K. C.

AU - Shimizu, H.

AU - Csikós, M.

AU - Sinemus, K.

AU - Beckert, W.

AU - Kähäri, V. M.

PY - 2008/4

Y1 - 2008/4

N2 - Background: Patients with recessive dystrophic epidermolysis bullosa (RDEB) have an increased risk of developing rapidly progressive and metastatic cutaneous squamous cell carcinomas (SCC). It is unclear why these SCC behave more aggressively than sporadic SCC. Matrix metalloproteinases (MMP) are a family of endopeptidases that contribute to growth, invasion and metastasis of SCC. The role of MMP in RDEB-associated SCC is not known. Objectives: To investigate the expression of MMP-7, MMP-13 and MMP-9 in RDEB-associated SCC in comparison with sporadic SCC and Bowen's disease. Methods: Immunohistochemical analysis of 25 RDEB-associated SCC, 61 sporadic SCC and 28 sporadic lesions of Bowen's disease was carried out using monoclonal antibodies for MMP-7, MMP-9, MMP-13 and E-cadherin and syndecan-1. Results: MMP-7 was detected in all RDEB-associated SCC, in tumour cells within the invasive edge, where E-cadherin and syndecan-1 were markedly diminished or absent. MMP-7 expression was also observed in 98% of sporadic SCC and in 68% of Bowen's diseases. MMP-7 staining was significantly stronger in RDEB-associated SCC than in sporadic SCC, and was most abundant in poorly differentiated tumours. MMP-13 was detected in tumour cells in 96% of RDEB-associated SCC and in all sporadic cutaneous SCC. MMP-9 was detected in the inflammatory cells in all SCC examined. Conclusions: These results identify MMP-7 and MMP-13 as tumour cell-specific markers for SCC progression and as potential therapeutic targets in RDEB-associated SCC. The pattern of immunolabelling suggests that MMP-7 may shed E-cadherin and syndecan-1 from the SCC cell surface.

AB - Background: Patients with recessive dystrophic epidermolysis bullosa (RDEB) have an increased risk of developing rapidly progressive and metastatic cutaneous squamous cell carcinomas (SCC). It is unclear why these SCC behave more aggressively than sporadic SCC. Matrix metalloproteinases (MMP) are a family of endopeptidases that contribute to growth, invasion and metastasis of SCC. The role of MMP in RDEB-associated SCC is not known. Objectives: To investigate the expression of MMP-7, MMP-13 and MMP-9 in RDEB-associated SCC in comparison with sporadic SCC and Bowen's disease. Methods: Immunohistochemical analysis of 25 RDEB-associated SCC, 61 sporadic SCC and 28 sporadic lesions of Bowen's disease was carried out using monoclonal antibodies for MMP-7, MMP-9, MMP-13 and E-cadherin and syndecan-1. Results: MMP-7 was detected in all RDEB-associated SCC, in tumour cells within the invasive edge, where E-cadherin and syndecan-1 were markedly diminished or absent. MMP-7 expression was also observed in 98% of sporadic SCC and in 68% of Bowen's diseases. MMP-7 staining was significantly stronger in RDEB-associated SCC than in sporadic SCC, and was most abundant in poorly differentiated tumours. MMP-13 was detected in tumour cells in 96% of RDEB-associated SCC and in all sporadic cutaneous SCC. MMP-9 was detected in the inflammatory cells in all SCC examined. Conclusions: These results identify MMP-7 and MMP-13 as tumour cell-specific markers for SCC progression and as potential therapeutic targets in RDEB-associated SCC. The pattern of immunolabelling suggests that MMP-7 may shed E-cadherin and syndecan-1 from the SCC cell surface.

KW - E-cadherin

KW - Epidermolysis bullosa

KW - Matrix metalloproteinase

KW - Squamous cell carcinoma

KW - Syndecan-1

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