Transformation of Medicago by Agrobacterium mediated gene transfer

Maria Deak, G. Kiss, Csaba Koncz, D. Dudits

Research output: Article

91 Citations (Scopus)

Abstract

Shoot segments of Medicago varia genotype A2 were co-cultivated with Agrobacterium tumefaciens strain bo42 carrying pGA471, a plasmid coding for the kanamycin resistant determinant as transferable positive selection marker in plant cells (An et al., 1985). Resistant plants were regenerated at high frequency from green calli developed on inoculated stem cuttings under kanamycin selection. DNA-DNA hybridization analysis showed the presence of the structural gene of the kanamycin resistant determinant in total DNA isolated from several independent transformants. All data presented clearly demonstrate the transfer, stable maintenance and functional expression of the kanamycin resistance marker in Medicago varia cells which retain their morphogenic property.

Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalPlant Cell Reports
Volume5
Issue number2
DOIs
Publication statusPublished - ápr. 1986

Fingerprint

Medicago
kanamycin
Agrobacterium
gene transfer
Medicago sativa nothosubsp. varia
structural genes
DNA
Agrobacterium radiobacter
nucleic acid hybridization
plasmids
callus
cells
stems
shoots
genotype

ASJC Scopus subject areas

  • Plant Science

Cite this

Transformation of Medicago by Agrobacterium mediated gene transfer. / Deak, Maria; Kiss, G.; Koncz, Csaba; Dudits, D.

In: Plant Cell Reports, Vol. 5, No. 2, 04.1986, p. 97-100.

Research output: Article

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