Transduction of Porcine Enteropathogenic Escherichia coli with a Derivative of a Shiga Toxin 2-Encoding Bacteriophage in a Porcine Ligated Ileal Loop System

István Tóth, Herbert Schmidt, Mohamed Dow, Anna Malik, Eric Oswald, Béla Nagy

Research output: Article

61 Citations (Scopus)

Abstract

In this study, we have investigated the ability of detoxified Shiga toxin (Stx)-converting bacteriophages Φ3538 (Δstx2::cat) (H. Schmidt et al., A ppl. Environ. Microbiol. 65:3855-3861, 1999) and H-19B::Tn10d-bla (D. W. Acheson et al., Infect. Immun. 66:4496-4498, 1998) to lysogenize enteropathogenic Escherichia coli (EPEC) strains in vivo. We were able to transduce the porcine EPEC strain 1390 (045) with Φ3538 (Δstx2::cat) in porcine ligated ileal loops but not the human EPEC prototype strain E234/69 (O127). Neither strain 1390 nor strain E2348/69 was lysogenized under these in vivo conditions when E. coli K-12 containing H-19B::Tn10d.bla was used as the stx1 phage donor. The repeated success in the in vivo transduction of an Stx2-encoding phage to a porcine EPEC strain in pig loops was in contrast to failures in the in vitro trials with these and other EPEC strains. These results indicate that in vivo conditions are more effective for transduction of Stx2-encoding phages than in vitro conditions.

Original languageEnglish
Pages (from-to)7242-7247
Number of pages6
JournalApplied and environmental microbiology
Volume69
Issue number12
DOIs
Publication statusPublished - dec. 1 2003

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ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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