Transcription specificity of the class Ib genes SLA-6, SLA-7 and SLA-8 of the swine major histocompatibility complex and comparison with class Ia genes

S. Kusza, L. Flori, Y. Gao, A. Teillaud, R. Hu, G. Lemonnier, Z. Bosze, E. Bourneuf, S. Vincent-Naulleau, C. Rogel-Gaillard

Research output: Article

5 Citations (Scopus)

Abstract

Our aim was to analyse the transcription levels of the three non-classical class Ib genes SLA-6, SLA-7 and SLA-8 of the swine major histocompatibility complex in various tissues and conditions and to compare them to the transcription levels of classical class Ia genes. Twenty-five adult tissues from two pig breeds, pig renal PK15 cells infected with the Pseudorabies virus, and peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide or a mixture of phorbol myristate acetate and ionomycin were included in our study. Relative transcription was quantified by quantitative real-time PCR. On average, in adult tissues and PBMCs and compared to SLA-6, the transcription level of SLA-Ia genes was 100-1000 times higher, the level of SLA-8 was 10-20 times higher, and that of SLA-7 was five times higher. Thus, SLA-8 is the most transcribed SLA-Ib gene, followed by the SLA-7 and SLA-6 genes. The highest transcription levels of SLA-Ib transcripts were found in the lymphoid organs, followed by the lung and the digestive tract. The tissue variability of expression levels was widest for the SLA-6 gene, with a 1:32 ratio between the lowest and highest levels in contrast to a 1:12 ratio for the SLA-7 and SLA-8 genes and a 1:16 ratio for the SLA-Ia genes. During PK-15 infection and PBMC stimulation, SLA-Ia and SLA-8 genes were downregulated, whereas SLA-6 and SLA-7 were upregulated, downregulated or not significantly modified. Our overall results confirm the tissue-wide transcription of the three SLA-Ib genes and suggest that they have complementary roles.

Original languageEnglish
Pages (from-to)510-520
Number of pages11
JournalAnimal Genetics
Volume42
Issue number5
DOIs
Publication statusPublished - okt. 2011

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics

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    Kusza, S., Flori, L., Gao, Y., Teillaud, A., Hu, R., Lemonnier, G., Bosze, Z., Bourneuf, E., Vincent-Naulleau, S., & Rogel-Gaillard, C. (2011). Transcription specificity of the class Ib genes SLA-6, SLA-7 and SLA-8 of the swine major histocompatibility complex and comparison with class Ia genes. Animal Genetics, 42(5), 510-520. https://doi.org/10.1111/j.1365-2052.2010.02170.x