Toxicokinetic study of a gastroprotective dose of capsaicin by HPLC-FLD method

Mónika Kuzma, Krisztina Fodor, Attila Almási, G. Mózsik, Tibor Past, P. Perjési

Research output: Article

Abstract

Background: A low dose of capsaicin and its natural homologs and analogs (capsaicinoids) have shown to prevent development of gastric mucosal damage of alcohol and non-steroid anti-inflammatory drugs. Based on this experimental observation, a drug development program has been initiated to develop per os applicable capsaicin containing drugs to eliminate gastrointestinal damage caused by non-steroid anti-inflammatory drugs. Methods: As a part of this program, a sensitive and selective reverse-phase high-performance liquid chromatography-based method with fluorescence detection has been developed for quantification of capsaicin and dihydrocapsaicin in experimental dog’s plasma. Results: The method was evaluated for a number of validation characteristics (selectivity, repeatability, and intermediate precision, LOD, LOQ, and calibration range). The limit of detection (LOD) was 2 ng/mL and the limit of quantification (LOQ) was 10 ng/mL for both capsaicin and dihydrocapsaicin. The method was used for analysis of capsaicin and dihydrocapsaicin in the plasma samples obtained after per os administration of low doses (0.1, 0.3, and 0.9 mg/kg bw) of Capsaicin Natural (USP 29) to the experimental animals. Conclusions: The obtained results indicated that the administered capsaicinoids did not reach the general circulation.

Original languageEnglish
Article number2848
JournalMolecules
Volume24
Issue number15
DOIs
Publication statusPublished - aug. 5 2019

Fingerprint

Capsaicin
drugs
High Pressure Liquid Chromatography
dosage
damage
Pharmaceutical Preparations
dogs
Limit of Detection
liquid chromatography
Anti-Inflammatory Agents
Plasmas
animals
alcohols
selectivity
High performance liquid chromatography
Reverse-Phase Chromatography
analogs
fluorescence
Calibration
Toxicokinetics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Toxicokinetic study of a gastroprotective dose of capsaicin by HPLC-FLD method. / Kuzma, Mónika; Fodor, Krisztina; Almási, Attila; Mózsik, G.; Past, Tibor; Perjési, P.

In: Molecules, Vol. 24, No. 15, 2848, 05.08.2019.

Research output: Article

Kuzma, Mónika ; Fodor, Krisztina ; Almási, Attila ; Mózsik, G. ; Past, Tibor ; Perjési, P. / Toxicokinetic study of a gastroprotective dose of capsaicin by HPLC-FLD method. In: Molecules. 2019 ; Vol. 24, No. 15.
@article{dfdab57cf1674bcf8082045c371076b0,
title = "Toxicokinetic study of a gastroprotective dose of capsaicin by HPLC-FLD method",
abstract = "Background: A low dose of capsaicin and its natural homologs and analogs (capsaicinoids) have shown to prevent development of gastric mucosal damage of alcohol and non-steroid anti-inflammatory drugs. Based on this experimental observation, a drug development program has been initiated to develop per os applicable capsaicin containing drugs to eliminate gastrointestinal damage caused by non-steroid anti-inflammatory drugs. Methods: As a part of this program, a sensitive and selective reverse-phase high-performance liquid chromatography-based method with fluorescence detection has been developed for quantification of capsaicin and dihydrocapsaicin in experimental dog’s plasma. Results: The method was evaluated for a number of validation characteristics (selectivity, repeatability, and intermediate precision, LOD, LOQ, and calibration range). The limit of detection (LOD) was 2 ng/mL and the limit of quantification (LOQ) was 10 ng/mL for both capsaicin and dihydrocapsaicin. The method was used for analysis of capsaicin and dihydrocapsaicin in the plasma samples obtained after per os administration of low doses (0.1, 0.3, and 0.9 mg/kg bw) of Capsaicin Natural (USP 29) to the experimental animals. Conclusions: The obtained results indicated that the administered capsaicinoids did not reach the general circulation.",
keywords = "Biotransformation, Capsaicin, Dihydrocapsaicin, Gastroprotection, High-performance liquid chromatography (HPLC), Non-steroid anti-inflammatory drugs",
author = "M{\'o}nika Kuzma and Krisztina Fodor and Attila Alm{\'a}si and G. M{\'o}zsik and Tibor Past and P. Perj{\'e}si",
year = "2019",
month = "8",
day = "5",
doi = "10.3390/molecules24152848",
language = "English",
volume = "24",
journal = "Molecules",
issn = "1420-3049",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "15",

}

TY - JOUR

T1 - Toxicokinetic study of a gastroprotective dose of capsaicin by HPLC-FLD method

AU - Kuzma, Mónika

AU - Fodor, Krisztina

AU - Almási, Attila

AU - Mózsik, G.

AU - Past, Tibor

AU - Perjési, P.

PY - 2019/8/5

Y1 - 2019/8/5

N2 - Background: A low dose of capsaicin and its natural homologs and analogs (capsaicinoids) have shown to prevent development of gastric mucosal damage of alcohol and non-steroid anti-inflammatory drugs. Based on this experimental observation, a drug development program has been initiated to develop per os applicable capsaicin containing drugs to eliminate gastrointestinal damage caused by non-steroid anti-inflammatory drugs. Methods: As a part of this program, a sensitive and selective reverse-phase high-performance liquid chromatography-based method with fluorescence detection has been developed for quantification of capsaicin and dihydrocapsaicin in experimental dog’s plasma. Results: The method was evaluated for a number of validation characteristics (selectivity, repeatability, and intermediate precision, LOD, LOQ, and calibration range). The limit of detection (LOD) was 2 ng/mL and the limit of quantification (LOQ) was 10 ng/mL for both capsaicin and dihydrocapsaicin. The method was used for analysis of capsaicin and dihydrocapsaicin in the plasma samples obtained after per os administration of low doses (0.1, 0.3, and 0.9 mg/kg bw) of Capsaicin Natural (USP 29) to the experimental animals. Conclusions: The obtained results indicated that the administered capsaicinoids did not reach the general circulation.

AB - Background: A low dose of capsaicin and its natural homologs and analogs (capsaicinoids) have shown to prevent development of gastric mucosal damage of alcohol and non-steroid anti-inflammatory drugs. Based on this experimental observation, a drug development program has been initiated to develop per os applicable capsaicin containing drugs to eliminate gastrointestinal damage caused by non-steroid anti-inflammatory drugs. Methods: As a part of this program, a sensitive and selective reverse-phase high-performance liquid chromatography-based method with fluorescence detection has been developed for quantification of capsaicin and dihydrocapsaicin in experimental dog’s plasma. Results: The method was evaluated for a number of validation characteristics (selectivity, repeatability, and intermediate precision, LOD, LOQ, and calibration range). The limit of detection (LOD) was 2 ng/mL and the limit of quantification (LOQ) was 10 ng/mL for both capsaicin and dihydrocapsaicin. The method was used for analysis of capsaicin and dihydrocapsaicin in the plasma samples obtained after per os administration of low doses (0.1, 0.3, and 0.9 mg/kg bw) of Capsaicin Natural (USP 29) to the experimental animals. Conclusions: The obtained results indicated that the administered capsaicinoids did not reach the general circulation.

KW - Biotransformation

KW - Capsaicin

KW - Dihydrocapsaicin

KW - Gastroprotection

KW - High-performance liquid chromatography (HPLC)

KW - Non-steroid anti-inflammatory drugs

UR - http://www.scopus.com/inward/record.url?scp=85070785310&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070785310&partnerID=8YFLogxK

U2 - 10.3390/molecules24152848

DO - 10.3390/molecules24152848

M3 - Article

C2 - 31387338

AN - SCOPUS:85070785310

VL - 24

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 15

M1 - 2848

ER -