Tissue transglutaminase ELISA positivity in autoimmune disease independent of gluten-sensitive disease

Miklós Sárdy, M. Csikós, Christof Geisen, Klaudia Preisz, Zoltán Kornseé, Erika Tomsits, Ulrich Töx, Nicolas Hunzelmann, Jörgen Wieslander, S. Kárpáti, Mats Paulsson, Neil Smyth

Research output: Article

30 Citations (Scopus)

Abstract

Background: Our aim was to understand why some sera from patients with a broad spectrum of autoimmune diseases or non-autoimmune diseases involving enhanced apoptosis, cell lysis and/or putative secondary autoimmune processes show reactions in the tissue transglutaminase (TGc) ELISA used for diagnosis of gluten-sensitive disease. Methods: Sera were compared from groups of patients with autoimmune diseases, diseases involving organ specific enhanced cell death, celiac disease or dermatitis herpetiformis, diseases of non-autoimmune origin, and a group without known disease. IgA antibodies against TGc were detected using human antigen (produced recombinantly in bacterial or human cells) in different systems (non-commercial ELISA with buffers of differing NaCl concentrations, and anti-TGc sandwich ELISA). Anti-gliadin and anti-endomysium antibodies were also determined. Results: Many sera from patients with autoimmune disorders gave a positive signal in the human TGc ELISAs. The signal appeared related to minor impurities in the recombinant human TGc used and to raised serum IgA antibody levels rather than to the occurrence of TGc specific antibodies in these patients. Conclusions: No association of anti-TGc Abs and autoimmune conditions independent of gluten-sensitive disease could be shown. Care should be taken to exclude copurification of chaperones, like heat shock protein 70, where preparing antigens for TGc ELISAs.

Original languageEnglish
Pages (from-to)126-135
Number of pages10
JournalClinica Chimica Acta
Volume376
Issue number1-2
DOIs
Publication statusPublished - febr. 1 2007

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Glutens
Autoimmune Diseases
Enzyme-Linked Immunosorbent Assay
Serum
Immunoglobulin A
Antibodies
Dermatitis Herpetiformis
Gliadin
Antigens
HSP70 Heat-Shock Proteins
Celiac Disease
Anti-Idiotypic Antibodies
Buffers
Dermatitis
Cell Death
transglutaminase 2
Apoptosis
Cell death
Cells
Impurities

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Tissue transglutaminase ELISA positivity in autoimmune disease independent of gluten-sensitive disease. / Sárdy, Miklós; Csikós, M.; Geisen, Christof; Preisz, Klaudia; Kornseé, Zoltán; Tomsits, Erika; Töx, Ulrich; Hunzelmann, Nicolas; Wieslander, Jörgen; Kárpáti, S.; Paulsson, Mats; Smyth, Neil.

In: Clinica Chimica Acta, Vol. 376, No. 1-2, 01.02.2007, p. 126-135.

Research output: Article

Sárdy, M, Csikós, M, Geisen, C, Preisz, K, Kornseé, Z, Tomsits, E, Töx, U, Hunzelmann, N, Wieslander, J, Kárpáti, S, Paulsson, M & Smyth, N 2007, 'Tissue transglutaminase ELISA positivity in autoimmune disease independent of gluten-sensitive disease', Clinica Chimica Acta, vol. 376, no. 1-2, pp. 126-135. https://doi.org/10.1016/j.cca.2006.08.006
Sárdy, Miklós ; Csikós, M. ; Geisen, Christof ; Preisz, Klaudia ; Kornseé, Zoltán ; Tomsits, Erika ; Töx, Ulrich ; Hunzelmann, Nicolas ; Wieslander, Jörgen ; Kárpáti, S. ; Paulsson, Mats ; Smyth, Neil. / Tissue transglutaminase ELISA positivity in autoimmune disease independent of gluten-sensitive disease. In: Clinica Chimica Acta. 2007 ; Vol. 376, No. 1-2. pp. 126-135.
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AU - Kornseé, Zoltán

AU - Tomsits, Erika

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