Tissue-specific signal(s) activate the promoter of a metallocarboxypeptidase inhibitor gene family in potato tuber and berry

Attila Molnár, Ágnes Lovas, Zsófia Bánfalvi, Lóránt Lakatos, Zsolt Polgár, Sándor Horváth

Research output: Article

13 Citations (Scopus)

Abstract

The molecular basis of the differential expression of the GM7-type metallocarboxypeptidase inhibitor (MCPI) genes in tuberizing (StMCPI) and non-tuberizing Solanum species (SbMCPI) was investigated. It was shown that the StMCPI is encoded by a gene family in Solanum tuberosum (potato), but SbMCPI might be a single-copy gene in the non-tuberizing species Solanum brevidens. The StMCPI promoter shows evolutionary relatedness to the S. brevidens-derived SbMCPI and to the fruit-specific tomato promoter 2A11. Both StMCPI and SbMCPI promoter regions were able to confer tuber- and berry-specific expression for the β-glucuronidase reporter gene in potato suggesting that the difference in MCPI gene expression is in trans regulatory factors between the tuberizing and the non-tuberizing Solanum species. The MCPI promoters did not respond to metabolic, environmental or hormonal signals in leaves. Thus, the MCPI genes are regulated in a different way than the other known tuber-specific genes and potentially are suitable for biotechnological application in potato to provide specific transgene expression in tuber and berry.

Original languageEnglish
Pages (from-to)301-311
Number of pages11
JournalPlant molecular biology
Volume46
Issue number3
DOIs
Publication statusPublished - aug. 1 2001

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ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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