Three-Dimensional Imaging of High Performance Liquid Chromatography Processes Using a Mini Positron Emission Tomograph

Eva Sarkadi-Priboczki, Ivan Valastyan, Karoly Brezovcsik, David Nagy, Gabor Opposits, Imre Lajtos, M. Emri, L. Balkay, J. Molnár

Research output: Article

1 Citation (Scopus)

Abstract

Positron emission tomography (PET) is a widely used technique for following radiotracer distribution, molecule specific binding or biochemical processes in living animals or in human body. Small animal PET cameras with high spatial resolution and high sensitivity are also useful, beyond their original pre-clinical application, in the following of surface chemical and the physicochemical processes. The purpose of this study was the introduction of the three-dimensional real-time PET based visualization method in separation processes. The separated tracer compounds can be followed and displayed in three dimension along chromatography column using the PET technique. We studied the separation of 11C-labeled methanol and 11C-labeled methyl iodide using reversed-phase high performance liquid chromatography (HPLC) on semi-preparative and analytical columns in order to determine the detection limits of our PET camera (MiniPET). The PET imaging is suitable for analyzing the column efficiency and the underlying processes in the liquid chromatography. Synthesis, separation and quality control of PET radiopharmaceuticals are performed in radiochemistry labs (in PET Centers) with the help of the liquid chromatography, therefore equipment, infrastructure and other lab conditions are usually available for researchers and for private companies to study chromatographic separation processes and to develop columns.

Original languageEnglish
Pages (from-to)9797-9802
Number of pages6
JournalChemistrySelect
Volume2
Issue number30
DOIs
Publication statusPublished - jan. 1 2017

Fingerprint

Positron emission tomography
High performance liquid chromatography
Positrons
Imaging techniques
Liquid chromatography
Animals
Cameras
Column chromatography
Radiopharmaceuticals
Quality control
Methanol
Visualization
Molecules

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Three-Dimensional Imaging of High Performance Liquid Chromatography Processes Using a Mini Positron Emission Tomograph. / Sarkadi-Priboczki, Eva; Valastyan, Ivan; Brezovcsik, Karoly; Nagy, David; Opposits, Gabor; Lajtos, Imre; Emri, M.; Balkay, L.; Molnár, J.

In: ChemistrySelect, Vol. 2, No. 30, 01.01.2017, p. 9797-9802.

Research output: Article

Sarkadi-Priboczki E, Valastyan I, Brezovcsik K, Nagy D, Opposits G, Lajtos I et al. Three-Dimensional Imaging of High Performance Liquid Chromatography Processes Using a Mini Positron Emission Tomograph. ChemistrySelect. 2017 jan. 1;2(30):9797-9802. https://doi.org/10.1002/slct.201701242
Sarkadi-Priboczki, Eva ; Valastyan, Ivan ; Brezovcsik, Karoly ; Nagy, David ; Opposits, Gabor ; Lajtos, Imre ; Emri, M. ; Balkay, L. ; Molnár, J. / Three-Dimensional Imaging of High Performance Liquid Chromatography Processes Using a Mini Positron Emission Tomograph. In: ChemistrySelect. 2017 ; Vol. 2, No. 30. pp. 9797-9802.
@article{50fe953b7f7c4786bc084c386dc259fd,
title = "Three-Dimensional Imaging of High Performance Liquid Chromatography Processes Using a Mini Positron Emission Tomograph",
abstract = "Positron emission tomography (PET) is a widely used technique for following radiotracer distribution, molecule specific binding or biochemical processes in living animals or in human body. Small animal PET cameras with high spatial resolution and high sensitivity are also useful, beyond their original pre-clinical application, in the following of surface chemical and the physicochemical processes. The purpose of this study was the introduction of the three-dimensional real-time PET based visualization method in separation processes. The separated tracer compounds can be followed and displayed in three dimension along chromatography column using the PET technique. We studied the separation of 11C-labeled methanol and 11C-labeled methyl iodide using reversed-phase high performance liquid chromatography (HPLC) on semi-preparative and analytical columns in order to determine the detection limits of our PET camera (MiniPET). The PET imaging is suitable for analyzing the column efficiency and the underlying processes in the liquid chromatography. Synthesis, separation and quality control of PET radiopharmaceuticals are performed in radiochemistry labs (in PET Centers) with the help of the liquid chromatography, therefore equipment, infrastructure and other lab conditions are usually available for researchers and for private companies to study chromatographic separation processes and to develop columns.",
keywords = "Compound band visualization, defective HPLC column monitoring, PET imaging, Positron emission tomography, Separation process follow-up",
author = "Eva Sarkadi-Priboczki and Ivan Valastyan and Karoly Brezovcsik and David Nagy and Gabor Opposits and Imre Lajtos and M. Emri and L. Balkay and J. Moln{\'a}r",
year = "2017",
month = "1",
day = "1",
doi = "10.1002/slct.201701242",
language = "English",
volume = "2",
pages = "9797--9802",
journal = "ChemistrySelect",
issn = "2365-6549",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "30",

}

TY - JOUR

T1 - Three-Dimensional Imaging of High Performance Liquid Chromatography Processes Using a Mini Positron Emission Tomograph

AU - Sarkadi-Priboczki, Eva

AU - Valastyan, Ivan

AU - Brezovcsik, Karoly

AU - Nagy, David

AU - Opposits, Gabor

AU - Lajtos, Imre

AU - Emri, M.

AU - Balkay, L.

AU - Molnár, J.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Positron emission tomography (PET) is a widely used technique for following radiotracer distribution, molecule specific binding or biochemical processes in living animals or in human body. Small animal PET cameras with high spatial resolution and high sensitivity are also useful, beyond their original pre-clinical application, in the following of surface chemical and the physicochemical processes. The purpose of this study was the introduction of the three-dimensional real-time PET based visualization method in separation processes. The separated tracer compounds can be followed and displayed in three dimension along chromatography column using the PET technique. We studied the separation of 11C-labeled methanol and 11C-labeled methyl iodide using reversed-phase high performance liquid chromatography (HPLC) on semi-preparative and analytical columns in order to determine the detection limits of our PET camera (MiniPET). The PET imaging is suitable for analyzing the column efficiency and the underlying processes in the liquid chromatography. Synthesis, separation and quality control of PET radiopharmaceuticals are performed in radiochemistry labs (in PET Centers) with the help of the liquid chromatography, therefore equipment, infrastructure and other lab conditions are usually available for researchers and for private companies to study chromatographic separation processes and to develop columns.

AB - Positron emission tomography (PET) is a widely used technique for following radiotracer distribution, molecule specific binding or biochemical processes in living animals or in human body. Small animal PET cameras with high spatial resolution and high sensitivity are also useful, beyond their original pre-clinical application, in the following of surface chemical and the physicochemical processes. The purpose of this study was the introduction of the three-dimensional real-time PET based visualization method in separation processes. The separated tracer compounds can be followed and displayed in three dimension along chromatography column using the PET technique. We studied the separation of 11C-labeled methanol and 11C-labeled methyl iodide using reversed-phase high performance liquid chromatography (HPLC) on semi-preparative and analytical columns in order to determine the detection limits of our PET camera (MiniPET). The PET imaging is suitable for analyzing the column efficiency and the underlying processes in the liquid chromatography. Synthesis, separation and quality control of PET radiopharmaceuticals are performed in radiochemistry labs (in PET Centers) with the help of the liquid chromatography, therefore equipment, infrastructure and other lab conditions are usually available for researchers and for private companies to study chromatographic separation processes and to develop columns.

KW - Compound band visualization

KW - defective HPLC column monitoring

KW - PET imaging

KW - Positron emission tomography

KW - Separation process follow-up

UR - http://www.scopus.com/inward/record.url?scp=85041708474&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041708474&partnerID=8YFLogxK

U2 - 10.1002/slct.201701242

DO - 10.1002/slct.201701242

M3 - Article

AN - SCOPUS:85041708474

VL - 2

SP - 9797

EP - 9802

JO - ChemistrySelect

JF - ChemistrySelect

SN - 2365-6549

IS - 30

ER -