The VP1 capsid protein of adeno-associated virus type 2 is carrying a phospholipase A2 domain required for virus infectivity

Anne Girod, Christiane E. Wobus, Z. Zádori, Martin Ried, Kristin Leike, Peter Tijssen, Jürgen A. Kleinschmidt, Michael Hallek

Research output: Article

186 Citations (Scopus)

Abstract

The unique region of the VP1 protein of parvoviruses was proposed to contain a parvoviral phospholipase A2 (pvPLA2) motif. Here, PLA2 activity is shown in the unique region of adeno-associated virus type 2 (AAV-2) VP1 when expressed as an isolated domain in bacteria. Mutations in this region of the capsid protein strongly reduced the infectivity of mutant virions in comparison to wild-type AAV-2. This correlated with effects on the activity of PLA2. The mutations had no influence on capsid assembly, packaging of viral genomes into particles or binding to and entry into HeLa cells. However, a delayed onset and reduced amount of early gene expression, as measured by Rep immunofluorescence, was observed. These results suggest that pvPLA2 activity is required for a step following perinuclear accumulation of virions but prior to early gene expression.

Original languageEnglish
Pages (from-to)973-978
Number of pages6
JournalJournal of General Virology
Volume83
Issue number5
Publication statusPublished - 2002

Fingerprint

Dependovirus
Phospholipases A2
Capsid Proteins
Virion
Viruses
Gene Expression
Parvovirus
Mutation
Viral Genome
Capsid
Product Packaging
HeLa Cells
Fluorescent Antibody Technique
Bacteria
Proteins

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

The VP1 capsid protein of adeno-associated virus type 2 is carrying a phospholipase A2 domain required for virus infectivity. / Girod, Anne; Wobus, Christiane E.; Zádori, Z.; Ried, Martin; Leike, Kristin; Tijssen, Peter; Kleinschmidt, Jürgen A.; Hallek, Michael.

In: Journal of General Virology, Vol. 83, No. 5, 2002, p. 973-978.

Research output: Article

Girod, A, Wobus, CE, Zádori, Z, Ried, M, Leike, K, Tijssen, P, Kleinschmidt, JA & Hallek, M 2002, 'The VP1 capsid protein of adeno-associated virus type 2 is carrying a phospholipase A2 domain required for virus infectivity', Journal of General Virology, vol. 83, no. 5, pp. 973-978.
Girod, Anne ; Wobus, Christiane E. ; Zádori, Z. ; Ried, Martin ; Leike, Kristin ; Tijssen, Peter ; Kleinschmidt, Jürgen A. ; Hallek, Michael. / The VP1 capsid protein of adeno-associated virus type 2 is carrying a phospholipase A2 domain required for virus infectivity. In: Journal of General Virology. 2002 ; Vol. 83, No. 5. pp. 973-978.
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