The synthesis and secretion of progesterone and 20α-hydroxy-Δ4-pregnen-3-one by the rabbit ovary at various intervals after a single injection of HCG

T. M. Mills, G. Telegdy, K. Savard

Research output: Article

10 Citations (Scopus)

Abstract

The effect of gonadotropic stimulation on rabbit ovarian synthesis and secretion of progesterone and 20α-hydroxy-Δ4-pregnen-3-one has been investigated. Ovarian venous blood samples and ovarian tissues were collected at various intervals after the intravenous administration of HCG. Ovarian progestin content was assessed directly, or tissues were sliced for incubation with acetate-1-14C in order to label the steroidal products. The results of these studies confirm the previously reported preovulatory rise and ovulatory fall in steroid secretion and extend the findings to include a similar pattern in the de novo synthesis of the progestins, non-polar lipids and sterols. Addition of HCG directly to incubations failed to restore the lost steroidogenic capacity of the ovaries obtained at the time of ovulation. Taken together, these results indicate that the cessation in steroidogenesis at the time of ovulation must involve more complex metabolic alterations than merely the depletion of the stores of steroidogenic cholesterol substrate as has been the suggested basis for this phenomenon.

Original languageEnglish
Pages (from-to)621-637
Number of pages17
JournalSteroids
Volume19
Issue number5
DOIs
Publication statusPublished - 1972

Fingerprint

Progestins
Ovulation
Progesterone
Ovary
Tissue
Rabbits
Injections
Sterols
Intravenous Administration
Labels
Acetates
Blood
Steroids
Cholesterol
Lipids
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Molecular Biology
  • Organic Chemistry
  • Pharmacology

Cite this

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title = "The synthesis and secretion of progesterone and 20α-hydroxy-Δ4-pregnen-3-one by the rabbit ovary at various intervals after a single injection of HCG",
abstract = "The effect of gonadotropic stimulation on rabbit ovarian synthesis and secretion of progesterone and 20α-hydroxy-Δ4-pregnen-3-one has been investigated. Ovarian venous blood samples and ovarian tissues were collected at various intervals after the intravenous administration of HCG. Ovarian progestin content was assessed directly, or tissues were sliced for incubation with acetate-1-14C in order to label the steroidal products. The results of these studies confirm the previously reported preovulatory rise and ovulatory fall in steroid secretion and extend the findings to include a similar pattern in the de novo synthesis of the progestins, non-polar lipids and sterols. Addition of HCG directly to incubations failed to restore the lost steroidogenic capacity of the ovaries obtained at the time of ovulation. Taken together, these results indicate that the cessation in steroidogenesis at the time of ovulation must involve more complex metabolic alterations than merely the depletion of the stores of steroidogenic cholesterol substrate as has been the suggested basis for this phenomenon.",
author = "Mills, {T. M.} and G. Telegdy and K. Savard",
year = "1972",
doi = "10.1016/S0039-128X(72)80028-X",
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TY - JOUR

T1 - The synthesis and secretion of progesterone and 20α-hydroxy-Δ4-pregnen-3-one by the rabbit ovary at various intervals after a single injection of HCG

AU - Mills, T. M.

AU - Telegdy, G.

AU - Savard, K.

PY - 1972

Y1 - 1972

N2 - The effect of gonadotropic stimulation on rabbit ovarian synthesis and secretion of progesterone and 20α-hydroxy-Δ4-pregnen-3-one has been investigated. Ovarian venous blood samples and ovarian tissues were collected at various intervals after the intravenous administration of HCG. Ovarian progestin content was assessed directly, or tissues were sliced for incubation with acetate-1-14C in order to label the steroidal products. The results of these studies confirm the previously reported preovulatory rise and ovulatory fall in steroid secretion and extend the findings to include a similar pattern in the de novo synthesis of the progestins, non-polar lipids and sterols. Addition of HCG directly to incubations failed to restore the lost steroidogenic capacity of the ovaries obtained at the time of ovulation. Taken together, these results indicate that the cessation in steroidogenesis at the time of ovulation must involve more complex metabolic alterations than merely the depletion of the stores of steroidogenic cholesterol substrate as has been the suggested basis for this phenomenon.

AB - The effect of gonadotropic stimulation on rabbit ovarian synthesis and secretion of progesterone and 20α-hydroxy-Δ4-pregnen-3-one has been investigated. Ovarian venous blood samples and ovarian tissues were collected at various intervals after the intravenous administration of HCG. Ovarian progestin content was assessed directly, or tissues were sliced for incubation with acetate-1-14C in order to label the steroidal products. The results of these studies confirm the previously reported preovulatory rise and ovulatory fall in steroid secretion and extend the findings to include a similar pattern in the de novo synthesis of the progestins, non-polar lipids and sterols. Addition of HCG directly to incubations failed to restore the lost steroidogenic capacity of the ovaries obtained at the time of ovulation. Taken together, these results indicate that the cessation in steroidogenesis at the time of ovulation must involve more complex metabolic alterations than merely the depletion of the stores of steroidogenic cholesterol substrate as has been the suggested basis for this phenomenon.

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JF - Steroids

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