The negative surface charge density of plasmalemma vesicles from wheat and oat roots

Ian M. Møller, Tomas Lundborg, Alajos Bérczi

Research output: Article

43 Citations (Scopus)

Abstract

Plasmalemma vesicles were isolated in a sucrose-containing medium from wheat and oat roots and the net negative surface charge density was determined with 9-aminoacridine fluorescence [Chow, W.S. and Barber, J. (1980) J. Biochem. Biophys. Methods 3, 173-185]. The outer surface of the vesicles (measured in the presence of sucrose) had densities of - 16 to - 20 mC·m-2 and -29 mC·m-2 for wheat and oat roots, respectively. The inner surface - presumed to be the cytoplasmic side and calculated from the values measured in the presence and absence of sucrose - was more negative, and its size depended on the salt status of the roots.

Original languageEnglish
Pages (from-to)181-185
Number of pages5
JournalFEBS letters
Volume167
Issue number1
DOIs
Publication statusPublished - febr. 13 1984

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ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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