The impact of extra-column band broadening on the chromatographic efficiency of 5cm long narrow-bore very efficient columns

Szabolcs Fekete, Jeno Fekete

Research output: Article

73 Citations (Scopus)

Abstract

Small columns packed with core-shell and sub-2 μm totally porous particles and monolith columns are very popular to conduct fast and efficient chromatographic separations. In order to carry out fast separations, short (2-5. cm) and narrow-bore (2-2.1. mm) columns are used to decrease the analyte retention volume. Beside the column efficiency, another significant issue is the extra-column band-spreading. The extra-column dispersion of a given LC system can dramatically decrease the performance of a small very efficient column. The aim of this study was to compare the extra-column peak variance contribution of several commercially available LC systems. The efficiency loss of three different type 5. cm long narrow bore, very efficient columns (monolith, sub-2 μm fully porous and sub-2 μm core-shell packing) as a function of extra-column peak variance, and as a function of flow rate and also kinetic plots (analysis time versus apparent column efficiency) are presented.

Original languageEnglish
Pages (from-to)5286-5291
Number of pages6
JournalJournal of Chromatography A
Volume1218
Issue number31
DOIs
Publication statusPublished - aug. 5 2011

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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