The heterogeneity of Fc receptors on human peripheral mononuclear blood cells

M. Sandor, G. Füst, G. A. Medgyesi, A. Erdei, J. Gergely

Research output: Article

22 Citations (Scopus)

Abstract

Fc-receptor positive peripheral blood mononuclear cells (PMBC) behaved differently after a temperature shift from 4 to 37°. Two types could be distinguished. Type I FcRI+ PMBC were transformed to FcR- while type II FcRII+ PMBC retained their FcR as measured by EA rosettes. The supernatants of the PMBC or the shed receptor purified on a Sepharose 4B-aggregated human IgG column blocked the EAR formation of FcRI+ PMBC but had no effect on EAR formation of FcRII+ PMBC. An investigation was made into the reason why rosette formation by FcRII+ cells could not be inhibited by FcRI. As an explanation, the role of differences in affinity or subclass specificity was excluded while the binding site(s) on the IgG molecule for FcRI and II proved to be different. The FcRII+ PMBC had a greater cellular avidity for sensitized erythrocytes than FcRI+ PMBC. The different states of FcR-s in the cell membrane are discussed as a possible source of heterogeneity.

Original languageEnglish
Pages (from-to)553-560
Number of pages8
JournalImmunology
Volume38
Issue number3
Publication statusPublished - 1979

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Fc Receptors
Blood Cells
Immunoglobulin G
Rosette Formation
Sepharose
Erythrocytes
Binding Sites
Cell Membrane
Temperature

ASJC Scopus subject areas

  • Immunology

Cite this

The heterogeneity of Fc receptors on human peripheral mononuclear blood cells. / Sandor, M.; Füst, G.; Medgyesi, G. A.; Erdei, A.; Gergely, J.

In: Immunology, Vol. 38, No. 3, 1979, p. 553-560.

Research output: Article

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