The establishment of MDMS-resistant cloned cell lines from in vitro cultured, MDMS-sensitive, Yoshida sarcoma cells

B. Szende, Margaret Fox

Research output: Article

4 Citations (Scopus)

Abstract

A cloned cell line established from an in vivo maintained Yoshida tumour, known to be sensitive to methylene dimethanesulphonate (MDMS), was subjected to selection in vitro by repeated treatments with MDMS. After six successive clonal isolations the resultant clone showed an increase in resistance to MDMS by a factor of 103. The cell lines selected in vitro for resistance to MDMS were not crossresistant to HN2, suggesting that sensitivity to bifunctional alkylating agents is controlled by at least two mutations. The biphasic nature of the dose-response curves even after successive cloning indicates some instability of factors determining resistance.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalChemico-Biological Interactions
Volume6
Issue number1
DOIs
Publication statusPublished - 1973

Fingerprint

Yoshida Sarcoma
Cells
Cell Line
R Factors
Cloning
Alkylating Agents
Organism Cloning
Tumors
Clone Cells
Mutation
In Vitro Techniques
methylene dimethanesulfonate
Neoplasms

ASJC Scopus subject areas

  • Toxicology

Cite this

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AU - Szende, B.

AU - Fox, Margaret

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AB - A cloned cell line established from an in vivo maintained Yoshida tumour, known to be sensitive to methylene dimethanesulphonate (MDMS), was subjected to selection in vitro by repeated treatments with MDMS. After six successive clonal isolations the resultant clone showed an increase in resistance to MDMS by a factor of 103. The cell lines selected in vitro for resistance to MDMS were not crossresistant to HN2, suggesting that sensitivity to bifunctional alkylating agents is controlled by at least two mutations. The biphasic nature of the dose-response curves even after successive cloning indicates some instability of factors determining resistance.

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