Abstract
The aim of this study was to gain detailed information about the diversity of planktonic bacterial communities of a worldwide special peat bedded natural thermal spa lake, and to reveal the effect of a lake wall reconstruction work. To compare the efficiency of different methods used for analyzing bacterial diversity, cultivation, molecular cloning and pyrosequencing were applied simultaneously. Despite the almost unchanged physical–chemical parameters and cell count values of lake water, remarkable differences were observed in the planktonic bacterial community structures during and after the reconstruction by all applied microbiological approaches. Rhodobacter sp. was found to be one of the most abundant community members during the works probably due to the sediment stirring effect of the reconstruction. Following the reconstruction higher diversity was detected than during the works by all approaches. Bacterial strains related to species Chryseobacterium and Exiguobacterium, furthermore sequences related to Arcobacter, Gemmobacter and MWH-UniP1 aquatic group were identified in the highest proportion at that time. Although the differences revealed by cultivation based and independent community structures were significant, only minor disparities were found by molecular cloning and next generation sequencing techniques.
| Original language | English |
|---|---|
| Pages (from-to) | 1077-1089 |
| Number of pages | 13 |
| Journal | Archives of Microbiology |
| Volume | 199 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - okt. 1 2017 |
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ASJC Scopus subject areas
- Microbiology
- Biochemistry
- Molecular Biology
- Genetics
Cite this
The effect of reconstruction works on planktonic bacterial diversity of a unique thermal lake revealed by cultivation, molecular cloning and next generation sequencing. / Krett, Gergely; Szabó, Attila; Felföldi, Tamás; Márialigeti, Károly; Borsodi, Andrea K.
In: Archives of Microbiology, Vol. 199, No. 8, 01.10.2017, p. 1077-1089.Research output: Article
}
TY - JOUR
T1 - The effect of reconstruction works on planktonic bacterial diversity of a unique thermal lake revealed by cultivation, molecular cloning and next generation sequencing
AU - Krett, Gergely
AU - Szabó, Attila
AU - Felföldi, Tamás
AU - Márialigeti, Károly
AU - Borsodi, Andrea K.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - The aim of this study was to gain detailed information about the diversity of planktonic bacterial communities of a worldwide special peat bedded natural thermal spa lake, and to reveal the effect of a lake wall reconstruction work. To compare the efficiency of different methods used for analyzing bacterial diversity, cultivation, molecular cloning and pyrosequencing were applied simultaneously. Despite the almost unchanged physical–chemical parameters and cell count values of lake water, remarkable differences were observed in the planktonic bacterial community structures during and after the reconstruction by all applied microbiological approaches. Rhodobacter sp. was found to be one of the most abundant community members during the works probably due to the sediment stirring effect of the reconstruction. Following the reconstruction higher diversity was detected than during the works by all approaches. Bacterial strains related to species Chryseobacterium and Exiguobacterium, furthermore sequences related to Arcobacter, Gemmobacter and MWH-UniP1 aquatic group were identified in the highest proportion at that time. Although the differences revealed by cultivation based and independent community structures were significant, only minor disparities were found by molecular cloning and next generation sequencing techniques.
AB - The aim of this study was to gain detailed information about the diversity of planktonic bacterial communities of a worldwide special peat bedded natural thermal spa lake, and to reveal the effect of a lake wall reconstruction work. To compare the efficiency of different methods used for analyzing bacterial diversity, cultivation, molecular cloning and pyrosequencing were applied simultaneously. Despite the almost unchanged physical–chemical parameters and cell count values of lake water, remarkable differences were observed in the planktonic bacterial community structures during and after the reconstruction by all applied microbiological approaches. Rhodobacter sp. was found to be one of the most abundant community members during the works probably due to the sediment stirring effect of the reconstruction. Following the reconstruction higher diversity was detected than during the works by all approaches. Bacterial strains related to species Chryseobacterium and Exiguobacterium, furthermore sequences related to Arcobacter, Gemmobacter and MWH-UniP1 aquatic group were identified in the highest proportion at that time. Although the differences revealed by cultivation based and independent community structures were significant, only minor disparities were found by molecular cloning and next generation sequencing techniques.
KW - Anthropogenic disturbance
KW - Cultivation
KW - Freshwater bacteria
KW - Molecular cloning
KW - Pyrosequencing
KW - Thermal lake
UR - http://www.scopus.com/inward/record.url?scp=85017646743&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85017646743&partnerID=8YFLogxK
U2 - 10.1007/s00203-017-1379-9
DO - 10.1007/s00203-017-1379-9
M3 - Article
C2 - 28417144
AN - SCOPUS:85017646743
VL - 199
SP - 1077
EP - 1089
JO - Archives of Microbiology
JF - Archives of Microbiology
SN - 0302-8933
IS - 8
ER -