The candidate oncogene CYP24A1: A potential biomarker for colorectal tumorigenesis

Henrik C. Horváth, P. Lakatos, J. Kósa, Krisztián Bácsi, Katalin Borka, Giovanna Bises, Thomas Nittke, Pamela A. Hershberger, G. Speer, Enikö Kállay

Research output: Article

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Abstract

The main autocrine/paracrine role of the active metabolite of vitamin D3, 1α,25-dihydroxyvitamin D3 (1,25-D3), is inhibition of cell growth and induction of cell differentiation and/or apoptosis. Synthesis and degradation of the secosteroid occurs not only in the kidney but also in normal tissue or malignant extrarenal tissues such as the colon. Because 25-hydroxyvitamin D3 24-hydroxylase (CYP24A1) is considered to be the main enzyme determining the biological half-life of 1,25-D3, we have examined expression of the CYP24A1 mRNA (by real-time RT-PCR) and protein (by immunohistochemistry) in normal human colon mucosa, colorectal adenomas, and adenocarcinomas in 111 patients. Although 76% of the normal and benign colonic tissue was either completely devoid of or expressed very low levels of CYP24A1, in the majority of the adenocarcinomas (69%), the enzyme was present at high concentrations. A parallel increased expression of the proliferation marker Ki-67 in the same samples suggests that overexpression of CYP24A1 reduced local 1,25-D3 availability, decreasing its antiproliferative effect.

Original languageEnglish
Pages (from-to)277-285
Number of pages9
JournalJournal of Histochemistry and Cytochemistry
Volume58
Issue number3
DOIs
Publication statusPublished - márc. 2010

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Oncogenes
Calcitriol
Carcinogenesis
Biomarkers
Secosteroids
Colon
Adenocarcinoma
Calcifediol
Cholecalciferol
Enzymes
Mixed Function Oxygenases
Adenoma
Half-Life
Real-Time Polymerase Chain Reaction
Cell Differentiation
Mucous Membrane
Immunohistochemistry
Apoptosis
Kidney
Messenger RNA

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

The candidate oncogene CYP24A1 : A potential biomarker for colorectal tumorigenesis. / Horváth, Henrik C.; Lakatos, P.; Kósa, J.; Bácsi, Krisztián; Borka, Katalin; Bises, Giovanna; Nittke, Thomas; Hershberger, Pamela A.; Speer, G.; Kállay, Enikö.

In: Journal of Histochemistry and Cytochemistry, Vol. 58, No. 3, 03.2010, p. 277-285.

Research output: Article

Horváth, Henrik C. ; Lakatos, P. ; Kósa, J. ; Bácsi, Krisztián ; Borka, Katalin ; Bises, Giovanna ; Nittke, Thomas ; Hershberger, Pamela A. ; Speer, G. ; Kállay, Enikö. / The candidate oncogene CYP24A1 : A potential biomarker for colorectal tumorigenesis. In: Journal of Histochemistry and Cytochemistry. 2010 ; Vol. 58, No. 3. pp. 277-285.
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