The bacterial attachment site of the temperate Rhizobium phage 16-3 overlaps the 3′ end of a putative proline tRNA gene

I. Papp, László Dorgai, Péter Papp, Erzsébet Jónás, F. Olasz, L. Orosz

Research output: Article

19 Citations (Scopus)

Abstract

Bacteriophage 16-3 inserts its genome into the chromosome of Rhizobium meliloti strain 41 (Rm41) by site-specific recombination. The DNA regions around the bacterial attachment site (attB) and one of the hybrid attachment sites bordering the integrated prophage (attL) were cloned and their nucleotide sequences determined. We demonstrated that the 51 by region, where the phage and bacterial DNA sequences are identical, is active as a target site for phage integration. Furthermore it overlaps the 3′ end of a putative proline tRNA gene. This gene shows 79% similartiy to the corresponding proline tRNA-like genomic target sequence of certain integrative plasmids in Actinomycetes.

Original languageEnglish
Pages (from-to)258-264
Number of pages7
JournalMGG Molecular & General Genetics
Volume240
Issue number2
DOIs
Publication statusPublished - aug. 1993

Fingerprint

Microbiological Attachment Sites
Rhizobium
Transfer RNA
Proline
Bacteriophages
Genes
Sinorhizobium meliloti
Prophages
Bacterial DNA
Actinobacteria
Genetic Recombination
Plasmids
Chromosomes
Genome
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

The bacterial attachment site of the temperate Rhizobium phage 16-3 overlaps the 3′ end of a putative proline tRNA gene. / Papp, I.; Dorgai, László; Papp, Péter; Jónás, Erzsébet; Olasz, F.; Orosz, L.

In: MGG Molecular & General Genetics, Vol. 240, No. 2, 08.1993, p. 258-264.

Research output: Article

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AU - Papp, I.

AU - Dorgai, László

AU - Papp, Péter

AU - Jónás, Erzsébet

AU - Olasz, F.

AU - Orosz, L.

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AB - Bacteriophage 16-3 inserts its genome into the chromosome of Rhizobium meliloti strain 41 (Rm41) by site-specific recombination. The DNA regions around the bacterial attachment site (attB) and one of the hybrid attachment sites bordering the integrated prophage (attL) were cloned and their nucleotide sequences determined. We demonstrated that the 51 by region, where the phage and bacterial DNA sequences are identical, is active as a target site for phage integration. Furthermore it overlaps the 3′ end of a putative proline tRNA gene. This gene shows 79% similartiy to the corresponding proline tRNA-like genomic target sequence of certain integrative plasmids in Actinomycetes.

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KW - Site-specific recombination

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