The atopic skin-like microenvironment modulates the T-cell-polarising cytokine production of myeloid dendritic cells, as determined by laser scanning cytometry

Georgina Nagy, Quang Minh Doan-Xuan, Krisztián Gáspár, Gábor Mócsai, A. Kapitány, D. Törőcsik, Z. Bacsó, E. Gyimesi, E. Remenyik, T. Bíró, A. Szegedi

Research output: Article

3 Citations (Scopus)

Abstract

Because it is not known exactly when or where myeloid dendritic cells (mDCs) acquire their atopic dermatitis (AD)-specific T-cell-polarising ability in patients with this condition, we used laser scanning cytometry (LSC) to determine whether isolated peripheral blood mDCs from AD patients differed from cells from controls in their cytokine expression profiles de novo and after stimulation with Staphylococcus enterotoxin B (SEB) and thymic stromal lymphopoietin (TSLP), which represents an AD-like microenvironment. Unstimulated mDCs from AD patients showed pluripotent T-cell-polarising capacity, and the surrounding skin microenvironment was essential for the distinctive, disease-specific activity of mDCs (Th2-Th22 bias). We also emphasise that LSC is an attractive technique to study the effect of new DC-targeted therapeutic modalities in AD.

Original languageEnglish
Pages (from-to)276-278
Number of pages3
JournalExperimental Dermatology
Volume23
Issue number4
DOIs
Publication statusPublished - 2014

Fingerprint

Laser Scanning Cytometry
T-cells
Myeloid Cells
Atopic Dermatitis
Dendritic Cells
Skin
Cytokines
Scanning
T-Lymphocytes
Lasers
Blood Cells
Blood

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Dermatology
  • Medicine(all)

Cite this

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title = "The atopic skin-like microenvironment modulates the T-cell-polarising cytokine production of myeloid dendritic cells, as determined by laser scanning cytometry",
abstract = "Because it is not known exactly when or where myeloid dendritic cells (mDCs) acquire their atopic dermatitis (AD)-specific T-cell-polarising ability in patients with this condition, we used laser scanning cytometry (LSC) to determine whether isolated peripheral blood mDCs from AD patients differed from cells from controls in their cytokine expression profiles de novo and after stimulation with Staphylococcus enterotoxin B (SEB) and thymic stromal lymphopoietin (TSLP), which represents an AD-like microenvironment. Unstimulated mDCs from AD patients showed pluripotent T-cell-polarising capacity, and the surrounding skin microenvironment was essential for the distinctive, disease-specific activity of mDCs (Th2-Th22 bias). We also emphasise that LSC is an attractive technique to study the effect of new DC-targeted therapeutic modalities in AD.",
keywords = "Atopic dermatitis, Laser scanning cytometry, Myeloid dendritic cells",
author = "Georgina Nagy and Doan-Xuan, {Quang Minh} and Kriszti{\'a}n G{\'a}sp{\'a}r and G{\'a}bor M{\'o}csai and A. Kapit{\'a}ny and D. T{\"o}rőcsik and Z. Bacs{\'o} and E. Gyimesi and E. Remenyik and T. B{\'i}r{\'o} and A. Szegedi",
year = "2014",
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language = "English",
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TY - JOUR

T1 - The atopic skin-like microenvironment modulates the T-cell-polarising cytokine production of myeloid dendritic cells, as determined by laser scanning cytometry

AU - Nagy, Georgina

AU - Doan-Xuan, Quang Minh

AU - Gáspár, Krisztián

AU - Mócsai, Gábor

AU - Kapitány, A.

AU - Törőcsik, D.

AU - Bacsó, Z.

AU - Gyimesi, E.

AU - Remenyik, E.

AU - Bíró, T.

AU - Szegedi, A.

PY - 2014

Y1 - 2014

N2 - Because it is not known exactly when or where myeloid dendritic cells (mDCs) acquire their atopic dermatitis (AD)-specific T-cell-polarising ability in patients with this condition, we used laser scanning cytometry (LSC) to determine whether isolated peripheral blood mDCs from AD patients differed from cells from controls in their cytokine expression profiles de novo and after stimulation with Staphylococcus enterotoxin B (SEB) and thymic stromal lymphopoietin (TSLP), which represents an AD-like microenvironment. Unstimulated mDCs from AD patients showed pluripotent T-cell-polarising capacity, and the surrounding skin microenvironment was essential for the distinctive, disease-specific activity of mDCs (Th2-Th22 bias). We also emphasise that LSC is an attractive technique to study the effect of new DC-targeted therapeutic modalities in AD.

AB - Because it is not known exactly when or where myeloid dendritic cells (mDCs) acquire their atopic dermatitis (AD)-specific T-cell-polarising ability in patients with this condition, we used laser scanning cytometry (LSC) to determine whether isolated peripheral blood mDCs from AD patients differed from cells from controls in their cytokine expression profiles de novo and after stimulation with Staphylococcus enterotoxin B (SEB) and thymic stromal lymphopoietin (TSLP), which represents an AD-like microenvironment. Unstimulated mDCs from AD patients showed pluripotent T-cell-polarising capacity, and the surrounding skin microenvironment was essential for the distinctive, disease-specific activity of mDCs (Th2-Th22 bias). We also emphasise that LSC is an attractive technique to study the effect of new DC-targeted therapeutic modalities in AD.

KW - Atopic dermatitis

KW - Laser scanning cytometry

KW - Myeloid dendritic cells

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DO - 10.1111/exd.12342

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VL - 23

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JO - Experimental Dermatology

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