The application of circular dichroism spectroscopy for the determination of metal ion speciation and coordination modes of peptide complexes

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Abstract

Copper(II) and nickel(II) complexes of the tripeptides containing methionyl residues (GlyGlyMet, GlyMetGly, MetGlyGly, MetMetAla, MetGlyMet and MetMetMet) and palladium(II) complexes of GlyMet, MetGly, GlyMetGly and GlyGlyMet were studied by CD spectroscopic measurements. Complex formation reactions between copper(II), nickel(II) and tripeptides were very similar to each other, [MH-2L]- being the major species in alkaline solutions. CD spectroscopy, however, was a very sensitive tool for the elucidation of small structural differences among peptide complexes, including the weak axial or equatorial coordinations of thioether residues. The significant differences in the spectral parameters of the species [CuL]+, [CuH-1L] and [CuH-2L]- made it also possible to calculate the stability constants and metal ion speciation from CD measurements.

Original languageEnglish
Pages (from-to)2149-2159
Number of pages11
JournalPolyhedron
Volume21
Issue number21
Publication statusPublished - szept. 15 2002

Fingerprint

Circular dichroism spectroscopy
Circular Dichroism
Nickel
Peptides
dichroism
peptides
Metal ions
Copper
Spectrum Analysis
metal ions
Metals
nickel
Ions
copper
Palladium
Sulfides
spectroscopy
palladium
Spectroscopy
ions

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry
  • Physical and Theoretical Chemistry
  • Materials Chemistry

Cite this

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title = "The application of circular dichroism spectroscopy for the determination of metal ion speciation and coordination modes of peptide complexes",
abstract = "Copper(II) and nickel(II) complexes of the tripeptides containing methionyl residues (GlyGlyMet, GlyMetGly, MetGlyGly, MetMetAla, MetGlyMet and MetMetMet) and palladium(II) complexes of GlyMet, MetGly, GlyMetGly and GlyGlyMet were studied by CD spectroscopic measurements. Complex formation reactions between copper(II), nickel(II) and tripeptides were very similar to each other, [MH-2L]- being the major species in alkaline solutions. CD spectroscopy, however, was a very sensitive tool for the elucidation of small structural differences among peptide complexes, including the weak axial or equatorial coordinations of thioether residues. The significant differences in the spectral parameters of the species [CuL]+, [CuH-1L] and [CuH-2L]- made it also possible to calculate the stability constants and metal ion speciation from CD measurements.",
keywords = "CD spectroscopy, Copper(II), Methionine, Nickel(II), Palladium(II), Peptides",
author = "K. Ősz and Be{\'a}ta B{\'o}ka and K. V{\'a}rnagy and I. S{\'o}v{\'a}g{\'o} and T. Kurt{\'a}n and S. Antus",
year = "2002",
month = "9",
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language = "English",
volume = "21",
pages = "2149--2159",
journal = "Polyhedron",
issn = "0277-5387",
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TY - JOUR

T1 - The application of circular dichroism spectroscopy for the determination of metal ion speciation and coordination modes of peptide complexes

AU - Ősz, K.

AU - Bóka, Beáta

AU - Várnagy, K.

AU - Sóvágó, I.

AU - Kurtán, T.

AU - Antus, S.

PY - 2002/9/15

Y1 - 2002/9/15

N2 - Copper(II) and nickel(II) complexes of the tripeptides containing methionyl residues (GlyGlyMet, GlyMetGly, MetGlyGly, MetMetAla, MetGlyMet and MetMetMet) and palladium(II) complexes of GlyMet, MetGly, GlyMetGly and GlyGlyMet were studied by CD spectroscopic measurements. Complex formation reactions between copper(II), nickel(II) and tripeptides were very similar to each other, [MH-2L]- being the major species in alkaline solutions. CD spectroscopy, however, was a very sensitive tool for the elucidation of small structural differences among peptide complexes, including the weak axial or equatorial coordinations of thioether residues. The significant differences in the spectral parameters of the species [CuL]+, [CuH-1L] and [CuH-2L]- made it also possible to calculate the stability constants and metal ion speciation from CD measurements.

AB - Copper(II) and nickel(II) complexes of the tripeptides containing methionyl residues (GlyGlyMet, GlyMetGly, MetGlyGly, MetMetAla, MetGlyMet and MetMetMet) and palladium(II) complexes of GlyMet, MetGly, GlyMetGly and GlyGlyMet were studied by CD spectroscopic measurements. Complex formation reactions between copper(II), nickel(II) and tripeptides were very similar to each other, [MH-2L]- being the major species in alkaline solutions. CD spectroscopy, however, was a very sensitive tool for the elucidation of small structural differences among peptide complexes, including the weak axial or equatorial coordinations of thioether residues. The significant differences in the spectral parameters of the species [CuL]+, [CuH-1L] and [CuH-2L]- made it also possible to calculate the stability constants and metal ion speciation from CD measurements.

KW - CD spectroscopy

KW - Copper(II)

KW - Methionine

KW - Nickel(II)

KW - Palladium(II)

KW - Peptides

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M3 - Article

AN - SCOPUS:0037106202

VL - 21

SP - 2149

EP - 2159

JO - Polyhedron

JF - Polyhedron

SN - 0277-5387

IS - 21

ER -