Temperature dependence of bovine serum albumin adsorption onto a poly(ethylene oxide)-grafted surface

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Abstract

The adsorption of bovine serum albumin (BSA) onto bare polyethylene (PE) and poly(ethylene oxide)-grafted PE particles was studied by the solution depletion method. Adsorption measurements were performed at different temperatures in the range of 25-65°C. The PE surface adsorbs high amounts of protein corresponding to a complete side-on monolayer in the temperature range 25-45°C. The considerable increase in the adsorbed amount and the shape of the isotherms at temperatures above 55°C indicate surface aggregation approaching the denaturation temperature of BSA. A lower degree of protein adsorption was obtained for PEO-grafted surfaces, and the amount of BSA adsorbed increases continuously with the temperature over the whole range studied. This is in accordance with the changing wettability of the PEO-grafted surface with temperature. The different temperature dependences of the adsorption behaviour found for PE and PEO-grafted surfaces show the joint influence of the protein properties in solution and the surface interactions on the adsorption.

Original languageEnglish
Pages (from-to)135-140
Number of pages6
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume76
Issue numberC
DOIs
Publication statusPublished - szept. 8 1993

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ASJC Scopus subject areas

  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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