Synthesis and studies on cell-penetrating peptides

Jean Remi Bertrand, Claude Malvy, Tiphanie Auguste, Gábor K. Tóth, Orsolya Kiss-Ivánkovits, Eszter Illyés, Miklós Hollósi, Sándor Bottka, Ilona Laczkó

Research output: Article

11 Citations (Scopus)

Abstract

The ability of different synthetic cell penetrating peptides, as Antennapedia (wild and Phe6 mutated penetratins), flock house virus, and integrin peptides to form complexes with a 25mer antisense oligonucleotide was compared and their conformation was determined by circular dichroism spectroscopy. The efficiency for oligonucleotide delivery into cells was measured using peptides labeled with a coumarin derivative showing blue fluorescence and the fluorescein-labeled antisense oligonucleotide showing green fluorescence. Fluorescence due to the excitation energy transfer confirmed the interaction of the antisense oligonucleotide and cell-penetrating peptides. The most efficient oligonucleotide delivery was found for penetratins. Comparison of the two types of penetratins shows that the wild-type penetratin proved to be more efficient than mutated penetratin. The paper also emphasizes that the attachment of a fluorescent label may have an effect on the conformation and flexibility of cell-penetrating peptides that must be taken into consideration when evaluating biological experiments.

Original languageEnglish
Pages (from-to)1307-1314
Number of pages8
JournalBioconjugate Chemistry
Volume20
Issue number7
DOIs
Publication statusPublished - júl. 15 2009

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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