Surprising lack of liposome-induced complement activation by artificial 1,3-diamidophospholipids in vitro

Simon Bugna, Marzia Buscema, Sofiya Matviykiv, Rudolf Urbanics, Andreas Weinberger, T. Mészáros, J. Szebeni, Andreas Zumbuehl, Till Saxer, Bert Müller

Research output: Article

11 Citations (Scopus)

Abstract

Cardio-vascular diseases are the main cause of death, emphasizing the need to improve patient treatment and survival. One therapeutic approach is a liposome-based drug carrier system specifically targeting constricted arteries. The recently discovered mechano-sensitive liposomes use hemodynamic shear-stress differences between healthy and constricted blood vessels as trigger for drug release. Liposomes are promising delivery containers but are being recognized as foreign by the immune system. Complement activation as essential factor of the recognition leads to adverse effects. Here, we tested complement activation by liposomes formulated from the artificial phospholipid Pad-PC-Pad in vitro. Surprisingly no complement activation was detected in human sera and porcine plasma. In in vivo experiments with three pigs, neither anaphylactic reactions nor other significant hemodynamic changes were observed even at comparably high liposome doses. The pilot study holds promise for an absence of complement-mediated adverse effects of Pad-PC-Pad liposomes in human.

Original languageEnglish
JournalNanomedicine: Nanotechnology, Biology, and Medicine
DOIs
Publication statusAccepted/In press - jún. 11 2015

Fingerprint

Liposomes
Complement Activation
Chemical activation
Hemodynamics
Swine
Patient treatment
Drug Carriers
Immune system
Phospholipids
Blood vessels
Anaphylaxis
Vascular Diseases
Containers
Blood Vessels
In Vitro Techniques
Shear stress
Cause of Death
Immune System
Arteries
Plasmas

ASJC Scopus subject areas

  • Molecular Medicine
  • Bioengineering
  • Biomedical Engineering
  • Materials Science(all)
  • Medicine (miscellaneous)
  • Pharmaceutical Science

Cite this

Surprising lack of liposome-induced complement activation by artificial 1,3-diamidophospholipids in vitro. / Bugna, Simon; Buscema, Marzia; Matviykiv, Sofiya; Urbanics, Rudolf; Weinberger, Andreas; Mészáros, T.; Szebeni, J.; Zumbuehl, Andreas; Saxer, Till; Müller, Bert.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, 11.06.2015.

Research output: Article

Bugna, Simon ; Buscema, Marzia ; Matviykiv, Sofiya ; Urbanics, Rudolf ; Weinberger, Andreas ; Mészáros, T. ; Szebeni, J. ; Zumbuehl, Andreas ; Saxer, Till ; Müller, Bert. / Surprising lack of liposome-induced complement activation by artificial 1,3-diamidophospholipids in vitro. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2015.
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