Suckling induced activation pattern in the brain of rat pups

János Barna, Eva Renner, Antónia Arszovszki, Melinda Cservenák, Z. Kovács, M. Palkóvits, Arpád Dobolyi

Research output: Article

1 Citation (Scopus)

Abstract

Objectives: The aim of the study was to understand the effects of suckling on the brain of the pups by mapping their brain activation pattern in response to suckling. Methods: The c-fos method was applied to identify activated neurons. Fasted rat pups were returned to their mothers for suckling and sacrificed 2 hours later for Fos immunohistochemistry. Double labeling was also performed to characterize some of the activated neurons. For comparison, another group of fasted pups were given dry food before Fos mapping. Results: After suckling, we found an increase in the number of Fos-immunoreactive neurons in the insular and somatosensory cortices, central amygdaloid nucleus (CAm), paraventricular (PVN) and supraoptic hypothalamic nuclei, lateral parabrachial nucleus (LPB), nucleus of the solitary tract (NTS), and the area postrema. Double labeling experiments demonstrated the activation of calcitonin gene-related peptide-ir (CGRP-ir) neurons in the LPB, corticotropin-releasing hormone-ir (CRH-ir) but not oxytocin-ir neurons in the PVN, and noradrenergic neurons in the NTS. In the CAm, Fos-ir neurons did not contain CRH but were apposed to CGRP-ir fiber terminals. Refeeding with dry food-induced Fos activation in all brain areas activated by suckling. The degree of activation was higher following dry food consumption than suckling in the insular cortex, and lower in the supraoptic nucleus and the NTS. Furthermore, the accumbens, arcuate, and dorsomedial hypothalamic nuclei, and the lateral hypothalamic area, which were not activated by suckling, showed activation by dry food. Discussion: Neurons in a number of brain areas are activated during suckling, and may participate in the signaling of satiety, taste perception, reward, food, and salt balance regulation.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalNutritional Neuroscience
DOIs
Publication statusAccepted/In press - febr. 9 2017

Fingerprint

Neurons
Brain
Food
Supraoptic Nucleus
Calcitonin Gene-Related Peptide
Cerebral Cortex
Dorsomedial Hypothalamic Nucleus
Taste Perception
Lateral Hypothalamic Area
Area Postrema
Brain Mapping
Adrenergic Neurons
Solitary Nucleus
Somatosensory Cortex
Paraventricular Hypothalamic Nucleus
Corticotropin-Releasing Hormone
Oxytocin
Reward
Salts
Immunohistochemistry

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Suckling induced activation pattern in the brain of rat pups. / Barna, János; Renner, Eva; Arszovszki, Antónia; Cservenák, Melinda; Kovács, Z.; Palkóvits, M.; Dobolyi, Arpád.

In: Nutritional Neuroscience, 09.02.2017, p. 1-11.

Research output: Article

Barna, János ; Renner, Eva ; Arszovszki, Antónia ; Cservenák, Melinda ; Kovács, Z. ; Palkóvits, M. ; Dobolyi, Arpád. / Suckling induced activation pattern in the brain of rat pups. In: Nutritional Neuroscience. 2017 ; pp. 1-11.
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AU - Renner, Eva

AU - Arszovszki, Antónia

AU - Cservenák, Melinda

AU - Kovács, Z.

AU - Palkóvits, M.

AU - Dobolyi, Arpád

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KW - Mother litter interaction

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KW - Rodent dam

KW - Satiety

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