Study of origin of cultivar "csaba Gyöngye" (Pearl of Csaba)

Pál Kozma, Andrea Balogh, E. Kiss, Zsolt Galli, Timea Koncz, L. Heszky

Research output: Conference contribution

2 Citations (Scopus)

Abstract

"Csaba gyöngye" (Pearl of Csaba) is the earliest ripening table grape cultivar in the Carpathian Basin, therefore its growing is not limited to Hungary, it is well-known and grown in every grape producing country of the world. According to several authors "Csaba gyöngye" was produced by crossing Bronnerstraube with Muscat Ottonel produced by János Mathiász. The seeds were presented to Adolf Stark, who was a great character of the first period of the Hungarian grape breeding and grew it in his garden in Békéscsaba. According to an other version: it was serendipitously discovered in 1904 growing in a garden in Béké scsaba, so its origin is not known. Because of this uncertainty about its "birth", the rather oral than written history of its origin, molecular approach should help to clarify the exact parentage of this cultivar. For this purpose RAPD and microsatellite analyses were applied with DNA templates isolated from the following cultivars: "Csaba gyöngye, Bronnerstraube, Muscat Ottonel, Heunisch weiss and Heunisch rot. These latter two cultivars were used as not "suspicious" types for sake of comparison. First 81 RAPD primers (OPERON and UBC) were tried for PCR amplification, 55 of them produced polymorphic pattern. Parallel with RAPD analyses microsatellite primers - including four of the six microsatellite primers recommended by the GENRES CT96 No 81 EU project - were applied in order to reveal polymorphisms and identities among the above mentioned cultivars. Based on the amplified DNA products showing polymorphism cluster analyses were carried out both for RAPD and microsatellite fragments. A dendrogram was constructed based on the similarity matrix data by applying the unweighted pair-group method with arithmetic averages (UPGMA) cluster analysis using the SYN-TAX program package for PC Version 5.0. For the estimation of genetic distances between cultivars the Simple Matching coefficient and Jaccard index were used. In case of RAPD data the least genetic distance was obtained between Csaba gyöngye and Muscat Ottonel (putative pollinator). Considering the dendrogram constructed by the Jaccards index: Csaba gyöngye, Muscat Ottonel clusters together. Bronnerstraube forms one cluster with of Heunisch rot, and Heunisch weiss clusters to the subclusters of the other cultivars. Dendrogram obtained with the same RAPD data but with Simple Matching Coefficient shows some differences: two main clusters are formed: the first consists of two subclusters: (a): Pearl of Csaba and Muscat Ottonel are in a common subcluster, to that (b) Bronnerstraube is joining. The second main cluster is formed by Heunish weiss and Heunisch rot. Further analyses - including more cultivars and new microsatelllites - are being carried out to explain the contradiction between the RAPD and microsatellite based results and to be able to prove the correct origin of Csaba gyöngye (Pearl of Csaba).

Original languageEnglish
Title of host publicationActa Horticulturae
Pages585-591
Number of pages7
Volume603
Publication statusPublished - ápr. 1 2003

Publication series

NameActa Horticulturae
Volume603
ISSN (Print)05677572

Fingerprint

pearls
cultivars
microsatellite repeats
gardens
genetic distance
grapes
genetic polymorphism
sake
table grapes
parentage
DNA
Hungary
pollinators
mouth
cluster analysis
ripening
uncertainty
basins
breeding
seeds

ASJC Scopus subject areas

  • Horticulture

Cite this

Kozma, P., Balogh, A., Kiss, E., Galli, Z., Koncz, T., & Heszky, L. (2003). Study of origin of cultivar "csaba Gyöngye" (Pearl of Csaba). In Acta Horticulturae (Vol. 603, pp. 585-591). (Acta Horticulturae; Vol. 603).

Study of origin of cultivar "csaba Gyöngye" (Pearl of Csaba). / Kozma, Pál; Balogh, Andrea; Kiss, E.; Galli, Zsolt; Koncz, Timea; Heszky, L.

Acta Horticulturae. Vol. 603 2003. p. 585-591 (Acta Horticulturae; Vol. 603).

Research output: Conference contribution

Kozma, P, Balogh, A, Kiss, E, Galli, Z, Koncz, T & Heszky, L 2003, Study of origin of cultivar "csaba Gyöngye" (Pearl of Csaba). in Acta Horticulturae. vol. 603, Acta Horticulturae, vol. 603, pp. 585-591.
Kozma P, Balogh A, Kiss E, Galli Z, Koncz T, Heszky L. Study of origin of cultivar "csaba Gyöngye" (Pearl of Csaba). In Acta Horticulturae. Vol. 603. 2003. p. 585-591. (Acta Horticulturae).
Kozma, Pál ; Balogh, Andrea ; Kiss, E. ; Galli, Zsolt ; Koncz, Timea ; Heszky, L. / Study of origin of cultivar "csaba Gyöngye" (Pearl of Csaba). Acta Horticulturae. Vol. 603 2003. pp. 585-591 (Acta Horticulturae).
@inproceedings{f76aafd0a5784f5f8663e7a790063c33,
title = "Study of origin of cultivar {"}csaba Gy{\"o}ngye{"} (Pearl of Csaba)",
abstract = "{"}Csaba gy{\"o}ngye{"} (Pearl of Csaba) is the earliest ripening table grape cultivar in the Carpathian Basin, therefore its growing is not limited to Hungary, it is well-known and grown in every grape producing country of the world. According to several authors {"}Csaba gy{\"o}ngye{"} was produced by crossing Bronnerstraube with Muscat Ottonel produced by J{\'a}nos Mathi{\'a}sz. The seeds were presented to Adolf Stark, who was a great character of the first period of the Hungarian grape breeding and grew it in his garden in B{\'e}k{\'e}scsaba. According to an other version: it was serendipitously discovered in 1904 growing in a garden in B{\'e}k{\'e} scsaba, so its origin is not known. Because of this uncertainty about its {"}birth{"}, the rather oral than written history of its origin, molecular approach should help to clarify the exact parentage of this cultivar. For this purpose RAPD and microsatellite analyses were applied with DNA templates isolated from the following cultivars: {"}Csaba gy{\"o}ngye, Bronnerstraube, Muscat Ottonel, Heunisch weiss and Heunisch rot. These latter two cultivars were used as not {"}suspicious{"} types for sake of comparison. First 81 RAPD primers (OPERON and UBC) were tried for PCR amplification, 55 of them produced polymorphic pattern. Parallel with RAPD analyses microsatellite primers - including four of the six microsatellite primers recommended by the GENRES CT96 No 81 EU project - were applied in order to reveal polymorphisms and identities among the above mentioned cultivars. Based on the amplified DNA products showing polymorphism cluster analyses were carried out both for RAPD and microsatellite fragments. A dendrogram was constructed based on the similarity matrix data by applying the unweighted pair-group method with arithmetic averages (UPGMA) cluster analysis using the SYN-TAX program package for PC Version 5.0. For the estimation of genetic distances between cultivars the Simple Matching coefficient and Jaccard index were used. In case of RAPD data the least genetic distance was obtained between Csaba gy{\"o}ngye and Muscat Ottonel (putative pollinator). Considering the dendrogram constructed by the Jaccards index: Csaba gy{\"o}ngye, Muscat Ottonel clusters together. Bronnerstraube forms one cluster with of Heunisch rot, and Heunisch weiss clusters to the subclusters of the other cultivars. Dendrogram obtained with the same RAPD data but with Simple Matching Coefficient shows some differences: two main clusters are formed: the first consists of two subclusters: (a): Pearl of Csaba and Muscat Ottonel are in a common subcluster, to that (b) Bronnerstraube is joining. The second main cluster is formed by Heunish weiss and Heunisch rot. Further analyses - including more cultivars and new microsatelllites - are being carried out to explain the contradiction between the RAPD and microsatellite based results and to be able to prove the correct origin of Csaba gy{\"o}ngye (Pearl of Csaba).",
keywords = "Bronnerstraube. Heunisch rot, Csaba Gy{\"o}ngye (Perle von Csaba, Heunisch weiss, Microsatellite and cluster analysis, Pearl of Csaba), Perle de Csaba, RAPD",
author = "P{\'a}l Kozma and Andrea Balogh and E. Kiss and Zsolt Galli and Timea Koncz and L. Heszky",
year = "2003",
month = "4",
day = "1",
language = "English",
isbn = "9789066059566",
volume = "603",
series = "Acta Horticulturae",
pages = "585--591",
booktitle = "Acta Horticulturae",

}

TY - GEN

T1 - Study of origin of cultivar "csaba Gyöngye" (Pearl of Csaba)

AU - Kozma, Pál

AU - Balogh, Andrea

AU - Kiss, E.

AU - Galli, Zsolt

AU - Koncz, Timea

AU - Heszky, L.

PY - 2003/4/1

Y1 - 2003/4/1

N2 - "Csaba gyöngye" (Pearl of Csaba) is the earliest ripening table grape cultivar in the Carpathian Basin, therefore its growing is not limited to Hungary, it is well-known and grown in every grape producing country of the world. According to several authors "Csaba gyöngye" was produced by crossing Bronnerstraube with Muscat Ottonel produced by János Mathiász. The seeds were presented to Adolf Stark, who was a great character of the first period of the Hungarian grape breeding and grew it in his garden in Békéscsaba. According to an other version: it was serendipitously discovered in 1904 growing in a garden in Béké scsaba, so its origin is not known. Because of this uncertainty about its "birth", the rather oral than written history of its origin, molecular approach should help to clarify the exact parentage of this cultivar. For this purpose RAPD and microsatellite analyses were applied with DNA templates isolated from the following cultivars: "Csaba gyöngye, Bronnerstraube, Muscat Ottonel, Heunisch weiss and Heunisch rot. These latter two cultivars were used as not "suspicious" types for sake of comparison. First 81 RAPD primers (OPERON and UBC) were tried for PCR amplification, 55 of them produced polymorphic pattern. Parallel with RAPD analyses microsatellite primers - including four of the six microsatellite primers recommended by the GENRES CT96 No 81 EU project - were applied in order to reveal polymorphisms and identities among the above mentioned cultivars. Based on the amplified DNA products showing polymorphism cluster analyses were carried out both for RAPD and microsatellite fragments. A dendrogram was constructed based on the similarity matrix data by applying the unweighted pair-group method with arithmetic averages (UPGMA) cluster analysis using the SYN-TAX program package for PC Version 5.0. For the estimation of genetic distances between cultivars the Simple Matching coefficient and Jaccard index were used. In case of RAPD data the least genetic distance was obtained between Csaba gyöngye and Muscat Ottonel (putative pollinator). Considering the dendrogram constructed by the Jaccards index: Csaba gyöngye, Muscat Ottonel clusters together. Bronnerstraube forms one cluster with of Heunisch rot, and Heunisch weiss clusters to the subclusters of the other cultivars. Dendrogram obtained with the same RAPD data but with Simple Matching Coefficient shows some differences: two main clusters are formed: the first consists of two subclusters: (a): Pearl of Csaba and Muscat Ottonel are in a common subcluster, to that (b) Bronnerstraube is joining. The second main cluster is formed by Heunish weiss and Heunisch rot. Further analyses - including more cultivars and new microsatelllites - are being carried out to explain the contradiction between the RAPD and microsatellite based results and to be able to prove the correct origin of Csaba gyöngye (Pearl of Csaba).

AB - "Csaba gyöngye" (Pearl of Csaba) is the earliest ripening table grape cultivar in the Carpathian Basin, therefore its growing is not limited to Hungary, it is well-known and grown in every grape producing country of the world. According to several authors "Csaba gyöngye" was produced by crossing Bronnerstraube with Muscat Ottonel produced by János Mathiász. The seeds were presented to Adolf Stark, who was a great character of the first period of the Hungarian grape breeding and grew it in his garden in Békéscsaba. According to an other version: it was serendipitously discovered in 1904 growing in a garden in Béké scsaba, so its origin is not known. Because of this uncertainty about its "birth", the rather oral than written history of its origin, molecular approach should help to clarify the exact parentage of this cultivar. For this purpose RAPD and microsatellite analyses were applied with DNA templates isolated from the following cultivars: "Csaba gyöngye, Bronnerstraube, Muscat Ottonel, Heunisch weiss and Heunisch rot. These latter two cultivars were used as not "suspicious" types for sake of comparison. First 81 RAPD primers (OPERON and UBC) were tried for PCR amplification, 55 of them produced polymorphic pattern. Parallel with RAPD analyses microsatellite primers - including four of the six microsatellite primers recommended by the GENRES CT96 No 81 EU project - were applied in order to reveal polymorphisms and identities among the above mentioned cultivars. Based on the amplified DNA products showing polymorphism cluster analyses were carried out both for RAPD and microsatellite fragments. A dendrogram was constructed based on the similarity matrix data by applying the unweighted pair-group method with arithmetic averages (UPGMA) cluster analysis using the SYN-TAX program package for PC Version 5.0. For the estimation of genetic distances between cultivars the Simple Matching coefficient and Jaccard index were used. In case of RAPD data the least genetic distance was obtained between Csaba gyöngye and Muscat Ottonel (putative pollinator). Considering the dendrogram constructed by the Jaccards index: Csaba gyöngye, Muscat Ottonel clusters together. Bronnerstraube forms one cluster with of Heunisch rot, and Heunisch weiss clusters to the subclusters of the other cultivars. Dendrogram obtained with the same RAPD data but with Simple Matching Coefficient shows some differences: two main clusters are formed: the first consists of two subclusters: (a): Pearl of Csaba and Muscat Ottonel are in a common subcluster, to that (b) Bronnerstraube is joining. The second main cluster is formed by Heunish weiss and Heunisch rot. Further analyses - including more cultivars and new microsatelllites - are being carried out to explain the contradiction between the RAPD and microsatellite based results and to be able to prove the correct origin of Csaba gyöngye (Pearl of Csaba).

KW - Bronnerstraube. Heunisch rot

KW - Csaba Gyöngye (Perle von Csaba

KW - Heunisch weiss

KW - Microsatellite and cluster analysis

KW - Pearl of Csaba)

KW - Perle de Csaba

KW - RAPD

UR - http://www.scopus.com/inward/record.url?scp=28644446694&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28644446694&partnerID=8YFLogxK

M3 - Conference contribution

SN - 9789066059566

VL - 603

T3 - Acta Horticulturae

SP - 585

EP - 591

BT - Acta Horticulturae

ER -