Study of dinuclear Rh(II) complexes of phenylalanine derivatives as potential anticancer agents by using X-ray fluorescence and X-ray absorption

Zsuzsa Majer, Szilvia Bosze, Ildikó Szabó, Victor G. Mihucz, Anikó Gaál, Gábor Szilvágyi, Giancarlo Pepponi, Florian Meirer, Peter Wobrauschek, Norbert Szoboszlai, Dieter Ingerle, Christina Streli

Research output: Article

7 Citations (Scopus)

Abstract

In vitro antitumor efficacy of several dinuclear bridgings and one chelate structure dirhodium(II) complex of N-protected phenylalanine derivatives were tested on HT-29 cells. The following synthesized and previously characterized complexes were applied in the present work: Rh2(OAc)4-n(O-Phe-Z)n (n=1-4, -O-Phe-Z=N-benzyloxycarbonyl-l-phenylalaninate), Rh2(OAc)4-n(O-Phe-Ac)n (n=1-4, -O-Phe-Ac=N-acetyl-l-phenylalaninate), Rh2(OAc)2(N-Me-D-Phe-O)2 corresponding to N-methyl-d-phenylalaninate as well as Rh2(OAc)4 (-OAc=acetate). Depending on the complex ligand type and its coordination number, the intracellular rhodium (Rh) content determined by total reflection X-ray fluorescence (TXRF) spectrometry in the HT-29 cells varied between 25 and 2500ng/106 cells. In vitro cytotoxicity and cytostatic evaluations of the compounds on HT-29 human cell culture were performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assay. Compared to Rh2(OAc)4, the Rh compounds containing one or two -O-Phe-Z moieties proved to be the most effective on the HT-29 cells. Moreover, synchrotron radiation TXRF-X-ray absorption near edge structure measurements suggested a change of the molecular symmetry of the dirhodium(II) center for the moderately in vitro cytotoxic, lipophilic l-phenylalanine derivative complexes, characterized also by low ligand exchange rate when they were studied on HT-29 cells.

Original languageEnglish
Number of pages1
JournalMicrochemical Journal
Volume120
DOIs
Publication statusPublished - jan. 1 2015

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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