Structure and expression of a hybrid proline-rich protein gene in the Solanaceous species, Solanum brevidens, Solanum tuberosum, and Lycopersicum esculentum

Lukáš Fischer, Agnes Lovas, Zdeněk Opatrý, Z. Bánfalvi

Research output: Article

5 Citations (Scopus)

Abstract

The full-length cDNA of a previously identified Solanum brevidens gene was isolated and characterised. DNA sequence analysis revealed an open reading frame that encodes a hybrid proline-rich cell wall protein of 407 amino acids. The putative protein was designated SbrPRP. The SbrPRP harbours three parts, an N-terminal signal peptide followed by a repetitive proline-rich domain and a cysteine-rich C-terminus resembling non-specific lipid-transfer proteins. The repetitive proline-rich domain contains two repeated motifs, PPHVKPPSTPK and PTPPIVSPP extended with TPKYP and TPKPPS motifs, respectively, at their N- or C-terminal. The SbrPRP gene of the non-tuberising Solarium species, Solanum brevidens, possesses highly homologous counterparts in the tuberising species, Solanum tuberosum (StPRP) and in the related species, Lycopersicum esculentum (TFM7). All three genes are present in single- or low copy number in the corresponding genome. Organ-specific expression of the genes, however, is different in the three solanaceous species.

Original languageEnglish
Pages (from-to)1271-1275
Number of pages5
JournalJournal of Plant Physiology
Volume159
Issue number11
Publication statusPublished - nov. 1 2002

Fingerprint

Solanum palustre
Solanum
Solanum lycopersicum
Lycopersicon esculentum
Solanum tuberosum
Proline
proline
Genes
Proteins
genes
proteins
Protein Sorting Signals
signal peptide
DNA Sequence Analysis
Cell Wall
Open Reading Frames
Cysteine
cysteine
open reading frames
sequence analysis

ASJC Scopus subject areas

  • Plant Science

Cite this

@article{235857898d4d4ee8b9ebbc38a653d573,
title = "Structure and expression of a hybrid proline-rich protein gene in the Solanaceous species, Solanum brevidens, Solanum tuberosum, and Lycopersicum esculentum",
abstract = "The full-length cDNA of a previously identified Solanum brevidens gene was isolated and characterised. DNA sequence analysis revealed an open reading frame that encodes a hybrid proline-rich cell wall protein of 407 amino acids. The putative protein was designated SbrPRP. The SbrPRP harbours three parts, an N-terminal signal peptide followed by a repetitive proline-rich domain and a cysteine-rich C-terminus resembling non-specific lipid-transfer proteins. The repetitive proline-rich domain contains two repeated motifs, PPHVKPPSTPK and PTPPIVSPP extended with TPKYP and TPKPPS motifs, respectively, at their N- or C-terminal. The SbrPRP gene of the non-tuberising Solarium species, Solanum brevidens, possesses highly homologous counterparts in the tuberising species, Solanum tuberosum (StPRP) and in the related species, Lycopersicum esculentum (TFM7). All three genes are present in single- or low copy number in the corresponding genome. Organ-specific expression of the genes, however, is different in the three solanaceous species.",
keywords = "Cell wall protein, Proline-rich protein, Solanaceae",
author = "Luk{\'a}š Fischer and Agnes Lovas and Zdeněk Opatr{\'y} and Z. B{\'a}nfalvi",
year = "2002",
month = "11",
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language = "English",
volume = "159",
pages = "1271--1275",
journal = "Journal of Plant Physiology",
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TY - JOUR

T1 - Structure and expression of a hybrid proline-rich protein gene in the Solanaceous species, Solanum brevidens, Solanum tuberosum, and Lycopersicum esculentum

AU - Fischer, Lukáš

AU - Lovas, Agnes

AU - Opatrý, Zdeněk

AU - Bánfalvi, Z.

PY - 2002/11/1

Y1 - 2002/11/1

N2 - The full-length cDNA of a previously identified Solanum brevidens gene was isolated and characterised. DNA sequence analysis revealed an open reading frame that encodes a hybrid proline-rich cell wall protein of 407 amino acids. The putative protein was designated SbrPRP. The SbrPRP harbours three parts, an N-terminal signal peptide followed by a repetitive proline-rich domain and a cysteine-rich C-terminus resembling non-specific lipid-transfer proteins. The repetitive proline-rich domain contains two repeated motifs, PPHVKPPSTPK and PTPPIVSPP extended with TPKYP and TPKPPS motifs, respectively, at their N- or C-terminal. The SbrPRP gene of the non-tuberising Solarium species, Solanum brevidens, possesses highly homologous counterparts in the tuberising species, Solanum tuberosum (StPRP) and in the related species, Lycopersicum esculentum (TFM7). All three genes are present in single- or low copy number in the corresponding genome. Organ-specific expression of the genes, however, is different in the three solanaceous species.

AB - The full-length cDNA of a previously identified Solanum brevidens gene was isolated and characterised. DNA sequence analysis revealed an open reading frame that encodes a hybrid proline-rich cell wall protein of 407 amino acids. The putative protein was designated SbrPRP. The SbrPRP harbours three parts, an N-terminal signal peptide followed by a repetitive proline-rich domain and a cysteine-rich C-terminus resembling non-specific lipid-transfer proteins. The repetitive proline-rich domain contains two repeated motifs, PPHVKPPSTPK and PTPPIVSPP extended with TPKYP and TPKPPS motifs, respectively, at their N- or C-terminal. The SbrPRP gene of the non-tuberising Solarium species, Solanum brevidens, possesses highly homologous counterparts in the tuberising species, Solanum tuberosum (StPRP) and in the related species, Lycopersicum esculentum (TFM7). All three genes are present in single- or low copy number in the corresponding genome. Organ-specific expression of the genes, however, is different in the three solanaceous species.

KW - Cell wall protein

KW - Proline-rich protein

KW - Solanaceae

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