Structure and biological activity of pathogen-like synthetic nanomedicines

Orsolya Lorincz, Eniko R. Toke, Eszter Somogyi, Ferenc Horkay, Preethi L. Chandran, Jack F. Douglas, János Szebeni, Julianna Lisziewicz

Research output: Article

22 Citations (Scopus)

Abstract

Here we characterize the structure, stability and intracellular mode of action of DermaVir nanomedicine that is under clinical development for the treatment of HIV/AIDS. This nanomedicine comprises pathogen-like pDNA/PEIm nanoparticles (NPs) having the structure and function resembling spherical viruses that naturally evolved to deliver nucleic acids to the cells. Atomic force microscopy demonstrated spherical 100 - 200 nm NPs with a smooth polymer surface protecting the pDNA in the core. Optical absorption determined both the NP structural stability and biological activity relevant to their ability to escape from the endosome and release the pDNA at the nucleus. Salt, pH and temperature influence nanomedicine shelf-life and intracellular stability. This approach facilitates the development of diverse polyplex nanomedicines where the delivered pDNA-expressed antigens induce immune responses to kill infected cells. From the Clinical Editor: The authors investigated DermaVir nanomedicine comprised of pathogen-like pDNA/PEIm nanoparticles with structure and function resembling spherical viruses. DermaVir delivery of pDNA expresses antigens that induce immune responses to kill HIV infected cells.

Original languageEnglish
Pages (from-to)497-506
Number of pages10
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume8
Issue number4
DOIs
Publication statusPublished - máj. 1 2012

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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  • Cite this

    Lorincz, O., Toke, E. R., Somogyi, E., Horkay, F., Chandran, P. L., Douglas, J. F., Szebeni, J., & Lisziewicz, J. (2012). Structure and biological activity of pathogen-like synthetic nanomedicines. Nanomedicine: Nanotechnology, Biology, and Medicine, 8(4), 497-506. https://doi.org/10.1016/j.nano.2011.07.013