Structural model of the 30S ribosomal subunit of Thermus Thermophilus with 35-angstrom spatial resolution. X-ray and neutron studies

Fan Lixin, D. I. Svergun, V. V. Volkov, V. L. Aksenov, S. Ch Agalarov, O. M. Selivanova, I. V. Shcherbakova, M. Koch, R. Gilles, A. Wiedenmann, R. May, I. N. Serdyuk

Research output: Article

Abstract

The two-phase model of the structure of the 30S ribosomal subunit of Thermus thermophilus has been obtained by the interpretation of the contrast variation data in X-ray and neutral small-angle scattering. The forms of the whole subunit and its region occupied by ribosomal RNA are presented by the envelope functions parametrized by spherical harmonics with a spatial resolution of 35 angstrom. The parametrization coefficients have been determined by the simulations approximation of the whole set of experimental data (14 scattering curves at different contrasts). The two-phase model for the 30S E. coli subunit has been used as the initial approximation. Comparison of the models shows that the main distinctions are in the spatial distribution of the protein component, whereas the forms of 16S RNA molecules for Thermus thermophilus and E. coli virtually do not differ at the given resolution level.

Original languageEnglish
Pages (from-to)1443-1456
Number of pages14
JournalSurface Investigation X-Ray, Synchrotron and Neutron Techniques
Volume14
Issue number11
Publication statusPublished - júl. 1 1999

ASJC Scopus subject areas

  • Surfaces, Coatings and Films

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    Lixin, F., Svergun, D. I., Volkov, V. V., Aksenov, V. L., Agalarov, S. C., Selivanova, O. M., Shcherbakova, I. V., Koch, M., Gilles, R., Wiedenmann, A., May, R., & Serdyuk, I. N. (1999). Structural model of the 30S ribosomal subunit of Thermus Thermophilus with 35-angstrom spatial resolution. X-ray and neutron studies. Surface Investigation X-Ray, Synchrotron and Neutron Techniques, 14(11), 1443-1456.