Structural characteristics influencing the carrier function of synthetic branched polypeptides based on poly[Lys-(dl-Ala)3)]backbone

É Rajnavölgyi, Á Lányi, F. Hudecz, I. Kurucz, K. Kiss, G. László, M. Szekerke, J. Gergely

Research output: Article

20 Citations (Scopus)

Abstract

Effective carrier function of selected representatives of new branched polypeptides covalently coupled with the synthetic monovalent hapten, oxazolone was studied. The effectiveness of oxazolone-synthetic polypeptide conjugates in inducing oxazolone-as well as carrier-specific antibody responses in inbred mice was compared to that of bovine serum albumin (BSA)- and KLH-oxazolone conjugates. The synthetic polypeptides, poly[Lys-(d-Leui-dl-Alam)] (d-LAK), LAK and FAK, as well as the common poly[Lys-(dl-Alam)](AK) core covalently coupled to oxazolone (Ox) induced a T cell-dependent antibody response when repeatedly administered with or without Freund's adjuvant in mice. This was evidenced by: the increasing titer of oxazolone-specific IgG during the course of the memory response; the appearance of all IgG subclasses; the effective oxazolone-specific priming by the conjugates; and the induction of an intense oxazolone- and carrier-specific DTH reaction. Although the oxazolone-specific antibody response was 10-100 times lower than that induced by KLH- or BSA-oxazolone conjugates, it was accompanied by a lower level or no detectable carrier-specific antibody response despite an effective carrier-specific T cell-mediated response. Significant differences were observed between the effectiveness of synthetic polypeptides used as carrier: highest oxazolone-specific antibody titers were observed using the AK, LAK and FAK conjugates. The intensity and specificity of the DTH reaction and antibody response induced by the carrier-oxazolone conjugates suggested that the distinct effectiveness of l- and d-amino acid-containing conjugates (LAK vs d-LAK and FAK vs d-FAK) was dependent on altered B cell recognition of the haptenic group. Circular dichroism (CD) spectra indicating different local orientation of oxazolone, when coupled to l or d side chain-terminating amino acids, support this suggestion.

Original languageEnglish
Pages (from-to)949-958
Number of pages10
JournalMolecular Immunology
Volume26
Issue number10
DOIs
Publication statusPublished - okt. 1989

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ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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