Strategies to obtain stable transgenic plants from non-embryogenic lines: Complementation of the nn1 mutation of the NORK gene in Medicago sativa MN1008

Ariana Perhald, G. Endré, Zoltan Kevei, G. Kiss, A. Kereszt

Research output: Article

1 Citation (Scopus)

Abstract

Strategies to introduce genes into non-embryogenic plants for complementation of a mutation are described and tested on tetraploid alfalfa (Medicago sativa). Genes conditioning embryogenic potential, a mutant phenotype, and a gene to complement the mutation can be combined using several different crossing and selection steps. In the successful strategy used here, the M. sativa genotype MnNC-1008(NN) carrying the recessive non-nodulating mutant allele nn1 was crossed with the highly embryogenic alfalfa line Regen S and embryogenic hybrid individuals were identified from the F1 progeny. After transformation of these hybrids with the wild-type gene (NORK), an F2 generation segregating for the mutation and transgene were produced. Plants homozygous for the mutant allele and carrying the wild-type NORK transgene could form root nodules after inoculation with Sinorhizobium meliloti demonstrating successful complementation of the nn1 mutation.

Original languageEnglish
Pages (from-to)799-806
Number of pages8
JournalPlant Cell Reports
Volume25
Issue number8
DOIs
Publication statusPublished - aug. 2006

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Medicago sativa
transgenic plants
mutation
mutants
transgenes
alfalfa
genes
alleles
Ensifer meliloti
root nodules
tetraploidy
complement
phenotype
genotype

ASJC Scopus subject areas

  • Plant Science

Cite this

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title = "Strategies to obtain stable transgenic plants from non-embryogenic lines: Complementation of the nn1 mutation of the NORK gene in Medicago sativa MN1008",
abstract = "Strategies to introduce genes into non-embryogenic plants for complementation of a mutation are described and tested on tetraploid alfalfa (Medicago sativa). Genes conditioning embryogenic potential, a mutant phenotype, and a gene to complement the mutation can be combined using several different crossing and selection steps. In the successful strategy used here, the M. sativa genotype MnNC-1008(NN) carrying the recessive non-nodulating mutant allele nn1 was crossed with the highly embryogenic alfalfa line Regen S and embryogenic hybrid individuals were identified from the F1 progeny. After transformation of these hybrids with the wild-type gene (NORK), an F2 generation segregating for the mutation and transgene were produced. Plants homozygous for the mutant allele and carrying the wild-type NORK transgene could form root nodules after inoculation with Sinorhizobium meliloti demonstrating successful complementation of the nn1 mutation.",
keywords = "Complementation, Embryogenicity, Medicago, Mutant, Nodulation, Stable transformation",
author = "Ariana Perhald and G. Endr{\'e} and Zoltan Kevei and G. Kiss and A. Kereszt",
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T1 - Strategies to obtain stable transgenic plants from non-embryogenic lines

T2 - Complementation of the nn1 mutation of the NORK gene in Medicago sativa MN1008

AU - Perhald, Ariana

AU - Endré, G.

AU - Kevei, Zoltan

AU - Kiss, G.

AU - Kereszt, A.

PY - 2006/8

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N2 - Strategies to introduce genes into non-embryogenic plants for complementation of a mutation are described and tested on tetraploid alfalfa (Medicago sativa). Genes conditioning embryogenic potential, a mutant phenotype, and a gene to complement the mutation can be combined using several different crossing and selection steps. In the successful strategy used here, the M. sativa genotype MnNC-1008(NN) carrying the recessive non-nodulating mutant allele nn1 was crossed with the highly embryogenic alfalfa line Regen S and embryogenic hybrid individuals were identified from the F1 progeny. After transformation of these hybrids with the wild-type gene (NORK), an F2 generation segregating for the mutation and transgene were produced. Plants homozygous for the mutant allele and carrying the wild-type NORK transgene could form root nodules after inoculation with Sinorhizobium meliloti demonstrating successful complementation of the nn1 mutation.

AB - Strategies to introduce genes into non-embryogenic plants for complementation of a mutation are described and tested on tetraploid alfalfa (Medicago sativa). Genes conditioning embryogenic potential, a mutant phenotype, and a gene to complement the mutation can be combined using several different crossing and selection steps. In the successful strategy used here, the M. sativa genotype MnNC-1008(NN) carrying the recessive non-nodulating mutant allele nn1 was crossed with the highly embryogenic alfalfa line Regen S and embryogenic hybrid individuals were identified from the F1 progeny. After transformation of these hybrids with the wild-type gene (NORK), an F2 generation segregating for the mutation and transgene were produced. Plants homozygous for the mutant allele and carrying the wild-type NORK transgene could form root nodules after inoculation with Sinorhizobium meliloti demonstrating successful complementation of the nn1 mutation.

KW - Complementation

KW - Embryogenicity

KW - Medicago

KW - Mutant

KW - Nodulation

KW - Stable transformation

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