Store-operated Ca2+ influx and subplasmalemmal mitochondria

Marek K. Korzeniowski, G. Szanda, Tamas Balla, A. Spät

Research output: Article

25 Citations (Scopus)

Abstract

Calcium depletion of the endoplasmic reticulum (ER) induces oligomerisation, puncta formation and translocation of the ER Ca2+ sensor proteins, STIM1 and -2 into plasma membrane (PM)-adjacent regions of the ER, where they activate the Orai1, -2 or -3 proteins present in the opposing PM. These proteins form ion channels through which store-operated Ca2+ influx (SOC) occurs. Calcium ions exert negative feed-back on SOC. Here we examined whether subplasmalemmal mitochondria, which reduce this feed-back by Ca2+ uptake, are located within or out of the high-Ca2+ microdomains (HCMDs) formed between the ER and plasmalemmal Orai1 channels. For this purpose, COS-7 cells were cotransfected with Orai1, STIM1 labelled with YFP or mRFP and the mitochondrially targeted Ca2+ sensitive fluorescent protein inverse-Pericam. Depletion of ER Ca2+ with ATP + thapsigargin (in Ca2+-free medium) induced the appearance of STIM1 puncta in the ≤100 nm wide subplasmalemmal space, as examined with TIRF. Mitochondria were located either in the gaps between STIM1-tagged puncta or in remote, STIM1-free regions. After addition of Ca2+ mitochondrial Ca2+ concentration increased irrespective of the mitochondrion-STIM1 distance. These observations indicate that mitochondria are exposed to Ca2+ diffused laterally from the HCMDs formed between the PM and the subplasmalemmal ER.

Original languageEnglish
Pages (from-to)49-55
Number of pages7
JournalCell Calcium
Volume46
Issue number1
DOIs
Publication statusPublished - júl. 2009

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Endoplasmic Reticulum
Mitochondria
Cell Membrane
Calcium
Proteins
Thapsigargin
COS Cells
Ion Channels
Adenosine Triphosphate
Ions

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Physiology

Cite this

Store-operated Ca2+ influx and subplasmalemmal mitochondria. / Korzeniowski, Marek K.; Szanda, G.; Balla, Tamas; Spät, A.

In: Cell Calcium, Vol. 46, No. 1, 07.2009, p. 49-55.

Research output: Article

Korzeniowski, Marek K. ; Szanda, G. ; Balla, Tamas ; Spät, A. / Store-operated Ca2+ influx and subplasmalemmal mitochondria. In: Cell Calcium. 2009 ; Vol. 46, No. 1. pp. 49-55.
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