Stereotaxic injection of IgG from patients with Alzheimer disease initiates injury of cholinergic neurons of the basal forebrain

József I. Engelhardt, Wei Dong Le, László Siklós, Izabella Obál, Krisztina Boda, Stanley H. Appel

Research output: Article

16 Citations (Scopus)

Abstract

Context: The participation of an immune/inflammatory process in the pathomechanism of sporadic Alzheimer disease (AD) has been suggested by evidence for activated microgila and the potential therapeutic benefit of anti-inflammatory medication. Objective: To define a possible role for IgG in the immune/inflammatory process of AD in humans, we assayed the ability of IgG samples from patients with AD to target the injury to cholinergic neurons in rat basal forebrain in vivo. Design: IgG purified from the serum or plasma from patients with AD and patients with other neurological disease who were used as control (DC) patients was injected stereotaxically into the medial septum of adult rats. Four weeks later coronal sections of the whole medial septum-diagonal bands of Broca region were immunostained for choline acetyltransferase (ChAT) to identify cholinergic neuronal cells. Setting: University medical centers. Patients: Blood samples were collected from 8 patients with probable and definite AD and from 6 age-matched DC patients. Main Outcome Measure: Detection of changes in the number of ChAT immunopositive cell profiles in sections and statistical evaluation Results: Four weeks after the injections, IgG samples from patients with AD significantly reduced the number of ChAT-immunostained cell profiles in the whole medial septum-diagonal bands of Broca region compared with IgGs from DC patients. Neither DC IgGs nor saline solution significantly decreased the number of ChAT-immunopositive neuronal cell profiles. Conclusion: Data document that IgG from patients with A.D can target a stereotaxically induced immmune/inflammatory injury to cholinergic neurons in the rat basal forebrain in vivo.

Original languageEnglish
Pages (from-to)681-686
Number of pages6
JournalArchives of Neurology
Volume57
Issue number5
Publication statusPublished - máj. 2000

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Cholinergic Neurons
Alzheimer Disease
Immunoglobulin G
Injections
Wounds and Injuries
Choline O-Acetyltransferase
Diagonal Band of Broca
Basal Forebrain
Alzheimer's Disease
Neuron
Aptitude
Sodium Chloride
Cholinergic Agents
Anti-Inflammatory Agents
Outcome Assessment (Health Care)

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "Stereotaxic injection of IgG from patients with Alzheimer disease initiates injury of cholinergic neurons of the basal forebrain",
abstract = "Context: The participation of an immune/inflammatory process in the pathomechanism of sporadic Alzheimer disease (AD) has been suggested by evidence for activated microgila and the potential therapeutic benefit of anti-inflammatory medication. Objective: To define a possible role for IgG in the immune/inflammatory process of AD in humans, we assayed the ability of IgG samples from patients with AD to target the injury to cholinergic neurons in rat basal forebrain in vivo. Design: IgG purified from the serum or plasma from patients with AD and patients with other neurological disease who were used as control (DC) patients was injected stereotaxically into the medial septum of adult rats. Four weeks later coronal sections of the whole medial septum-diagonal bands of Broca region were immunostained for choline acetyltransferase (ChAT) to identify cholinergic neuronal cells. Setting: University medical centers. Patients: Blood samples were collected from 8 patients with probable and definite AD and from 6 age-matched DC patients. Main Outcome Measure: Detection of changes in the number of ChAT immunopositive cell profiles in sections and statistical evaluation Results: Four weeks after the injections, IgG samples from patients with AD significantly reduced the number of ChAT-immunostained cell profiles in the whole medial septum-diagonal bands of Broca region compared with IgGs from DC patients. Neither DC IgGs nor saline solution significantly decreased the number of ChAT-immunopositive neuronal cell profiles. Conclusion: Data document that IgG from patients with A.D can target a stereotaxically induced immmune/inflammatory injury to cholinergic neurons in the rat basal forebrain in vivo.",
author = "Engelhardt, {J{\'o}zsef I.} and Le, {Wei Dong} and L{\'a}szl{\'o} Sikl{\'o}s and Izabella Ob{\'a}l and Krisztina Boda and Appel, {Stanley H.}",
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T1 - Stereotaxic injection of IgG from patients with Alzheimer disease initiates injury of cholinergic neurons of the basal forebrain

AU - Engelhardt, József I.

AU - Le, Wei Dong

AU - Siklós, László

AU - Obál, Izabella

AU - Boda, Krisztina

AU - Appel, Stanley H.

PY - 2000/5

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N2 - Context: The participation of an immune/inflammatory process in the pathomechanism of sporadic Alzheimer disease (AD) has been suggested by evidence for activated microgila and the potential therapeutic benefit of anti-inflammatory medication. Objective: To define a possible role for IgG in the immune/inflammatory process of AD in humans, we assayed the ability of IgG samples from patients with AD to target the injury to cholinergic neurons in rat basal forebrain in vivo. Design: IgG purified from the serum or plasma from patients with AD and patients with other neurological disease who were used as control (DC) patients was injected stereotaxically into the medial septum of adult rats. Four weeks later coronal sections of the whole medial septum-diagonal bands of Broca region were immunostained for choline acetyltransferase (ChAT) to identify cholinergic neuronal cells. Setting: University medical centers. Patients: Blood samples were collected from 8 patients with probable and definite AD and from 6 age-matched DC patients. Main Outcome Measure: Detection of changes in the number of ChAT immunopositive cell profiles in sections and statistical evaluation Results: Four weeks after the injections, IgG samples from patients with AD significantly reduced the number of ChAT-immunostained cell profiles in the whole medial septum-diagonal bands of Broca region compared with IgGs from DC patients. Neither DC IgGs nor saline solution significantly decreased the number of ChAT-immunopositive neuronal cell profiles. Conclusion: Data document that IgG from patients with A.D can target a stereotaxically induced immmune/inflammatory injury to cholinergic neurons in the rat basal forebrain in vivo.

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