Stepwise gradient separation and quantification of dansylated biogenic amines in vegetables using personal OPLC instrument

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A new efficient OPLC method has been developed for the separation of seven biogenic amines (agmatine, spermine, spermidine, putrescine, cadaverine, histamine, tyramine, and NH3 as interfering component) in foods. The separation of the dansylated derivatives of the amines was performed on 20 cm x 20 cm silica gel HPTLC plates with stepwise gradient elution using the Personal OPLC BS 50 overpressured-layer chromatograph. The first solvent system was n-hexane-n-butanol-triethylamine, 90 + 10 + 9.1 (v/v) and the second was n-hexane-n-butanol, 8 + 2 (v/v). Approximately 26 min were needed for simultaneous separation of 14 samples; the detection limit was 2.5-15 ng spot-1, approximately 100 times lower than the critical toxic limit in foods. The method has been used successfully to screen for biogenic amines in samples of different vegetables.

Original languageEnglish
Pages (from-to)43-46
Number of pages4
JournalJournal of Planar Chromatography - Modern TLC
Issue number1
Publication statusPublished - jan. 1 1998


ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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