Stable incorporation of genetic material into the chromosome of Rhizobium meliloti 41: construction of an integrative vector system

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Abstract

An integrative vector system has been developed from the site-specific recombination elements of temperate phage 16-3. The system can be used for highly efficient stable introduction of genetic material into the chromosome of the symbiotic nitrogen-fixing organism, Rhizobium meliloti 41 (Rm41) at the attB site. Vectors carrying the phage-bome attachment site were constructed, and helper phages providing the site-specific recombination functions in trans were isolated. Other possible applications of the system are discussed.

Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalGene
Volume119
Issue number1
DOIs
Publication statusPublished - szept. 21 1992

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ASJC Scopus subject areas

  • Genetics

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