Mátrix-asszisztált lézer deszorpciós, ionizációs, repülési ido mérésén alapuló tömegspektrometria speciális alkalmazása a klinikai mikrobiológiai diagnosztika területén

E. Nagy, Marianna Ábrók, Noémi Bartha, László Bereczki, Emese Juhász, G. Kardos, Katalin Kristóf, Cecilia Miszti, E. Urbán

Research output: Article

3 Citations (Scopus)

Abstract

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry as a new possibility for rapid identification of bacteria and fungi revolutionized the clinical microbiological diagnostics. It has an extreme importance in the routine microbiological laboratories, as identification of the pathogenic species rapidly will influence antibiotic selection before the final determination of antibiotic resistance of the isolate. The classical methods for identification of bacteria or fungi, based on biochemical tests, are influenced by many environmental factors. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry is a rapid method which is able to identify a great variety of the isolated bacteria and fungi based on the composition of conserved ribosomal proteins. Recently several other applications of the method have also been investigated such as direct identification of pathogens from the positive blood cultures. There are possibilities to identify bacteria from the urine samples in urinary tract infection or from other sterile body fluids. Using selective enrichment broth Salmonella sp from the stool samples can be identified more rapidly, too. The extended spectrum beta-lactamase or carbapenemase production of the isolated bacteria can be also detected by this method helping the antibiotic selection in some cases. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry based methods are suitable to investigate changes in deoxyribonucleic acid or ribonucleic acid, to carry out rapid antibiotic resistance determination or other proteomic analysis. The aim of this paper is to give an overview about present possibilities of using this technique in the clinical microbiological routine procedures.

Original languageHungarian
Pages (from-to)1495-1503
Number of pages9
JournalOrvosi Hetilap
Volume155
Issue number38
DOIs
Publication statusPublished - szept. 1 2014

Fingerprint

Mass Spectrometry
Lasers
Bacteria
Fungi
Microbial Drug Resistance
Anti-Bacterial Agents
Ribosomal Proteins
Body Fluids
beta-Lactamases
Urinary Tract Infections
Salmonella
Proteomics
Urine
RNA
DNA

Keywords

  • antibiotic resistance determination
  • MALDI-TOF MS
  • species level identification
  • typing

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Mátrix-asszisztált lézer deszorpciós, ionizációs, repülési ido mérésén alapuló tömegspektrometria speciális alkalmazása a klinikai mikrobiológiai diagnosztika területén. / Nagy, E.; Ábrók, Marianna; Bartha, Noémi; Bereczki, László; Juhász, Emese; Kardos, G.; Kristóf, Katalin; Miszti, Cecilia; Urbán, E.

In: Orvosi Hetilap, Vol. 155, No. 38, 01.09.2014, p. 1495-1503.

Research output: Article

Nagy, E. ; Ábrók, Marianna ; Bartha, Noémi ; Bereczki, László ; Juhász, Emese ; Kardos, G. ; Kristóf, Katalin ; Miszti, Cecilia ; Urbán, E. / Mátrix-asszisztált lézer deszorpciós, ionizációs, repülési ido mérésén alapuló tömegspektrometria speciális alkalmazása a klinikai mikrobiológiai diagnosztika területén. In: Orvosi Hetilap. 2014 ; Vol. 155, No. 38. pp. 1495-1503.
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