Five nutrient media were tested for expression of morphological pathway in Datura innoxia callus cultures. Regenerants-rooted plants and non-rooted shoots obtained from three subsequent subcultures of callus cultures were assayed for tropane alkaloid content and composition. Although no wide variation of bio-chemical traits of regenerants was observed. For a given number of plants decrease of hyoscyamine in their roots was detected. Alkaloid content and composition of upper parts of plants having roots and shoots or lacking roots was studied-receiving an idea about biosynthesis of these organs.
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