SMARCB1 expression in epithelioid sarcoma is regulated by miR-206, miR-381, and miR-671-5p on Both mRNA and protein levels

Gergo Papp, Thomas Krausz, Thomas P. Stricker, M. Szendrői, Z. Sápi

Research output: Article

35 Citations (Scopus)

Abstract

Proximal type epithelioid sarcoma shares similarities with malignant rhabdoid tumor, including the lack of nuclear immunoreactivity of SMARCB1. Biallelic mutation of SMARCB1 has been convincingly established as the cause of loss of protein expression in rhabdoid tumor, but the cause in epithelioid sarcoma remains unknown. In our previous work, we demonstrated that DNA hypermethylation and post-translational modification mechanisms were not involved. In this current work, we explored the hypothesis that miRNAs regulate SMARCB1 gene expression in epithelioid sarcomas. In silico target prediction analysis revealed eight candidate miRNAs, and quantitative PCR-in 32 formalin-fixed, paraffin-embedded tumor samples comprising 30 epithelioid sarcomas and two malignant rhabdoid tumors-demonstrated significant (P

Original languageEnglish
Pages (from-to)168-176
Number of pages9
JournalGenes Chromosomes and Cancer
Volume53
Issue number2
DOIs
Publication statusPublished - febr. 2014

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Rhabdoid Tumor
Sarcoma
Messenger RNA
MicroRNAs
Proteins
Post Translational Protein Processing
Computer Simulation
Paraffin
Formaldehyde
Gene Expression
Polymerase Chain Reaction
Mutation
DNA
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

SMARCB1 expression in epithelioid sarcoma is regulated by miR-206, miR-381, and miR-671-5p on Both mRNA and protein levels. / Papp, Gergo; Krausz, Thomas; Stricker, Thomas P.; Szendrői, M.; Sápi, Z.

In: Genes Chromosomes and Cancer, Vol. 53, No. 2, 02.2014, p. 168-176.

Research output: Article

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