Small RNA NGS revealed the presence of cherry virus A and little cherry virus 1 on apricots in hungary

Dániel Baráth, Nikoletta Jaksa-Czotter, János Molnár, Tünde Varga, Júlia Balássy, Luca Krisztina Szabó, Zoltán Kirilla, G. Tusnády, Éva Preininger, E. Várallyay

Research output: Article

2 Citations (Scopus)

Abstract

Fruit trees, such as apricot trees, are constantly exposed to the attack of viruses. As they are propagated in a vegetative way, this risk is present not only in the field, where they remain for decades, but also during their propagation. Metagenomic diagnostic methods, based on next generation sequencing (NGS), offer unique possibilities to reveal all the present pathogens in the investigated sample. Using NGS of small RNAs, a special field of these techniques, we tested leaf samples of different varieties of apricot originating from an isolator house or open field stock nursery. As a result, we identified Cherry virus A (CVA) and little cherry virus 1 (LChV-1) for the first time in Hungary. The NGS results were validated by RT-PCR and also by Northern blot in the case of CVA. Cloned and Sanger sequenced viral-specific PCR products enabled us to investigate their phylogenetic relationships. However, since these pathogens have not been described in our country before, their role in symptom development and modification during co-infection with other viruses requires further investigation.

Original languageEnglish
Article number318
JournalViruses
Volume10
Issue number6
DOIs
Publication statusPublished - jún. 11 2018

Fingerprint

Hungary
RNA
Viruses
RNA Sequence Analysis
Metagenomics
Polymerase Chain Reaction
Nurseries
Coinfection
Northern Blotting
Fruit
Prunus armeniaca

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Baráth, D., Jaksa-Czotter, N., Molnár, J., Varga, T., Balássy, J., Szabó, L. K., ... Várallyay, E. (2018). Small RNA NGS revealed the presence of cherry virus A and little cherry virus 1 on apricots in hungary. Viruses, 10(6), [318]. https://doi.org/10.3390/v10060318

Small RNA NGS revealed the presence of cherry virus A and little cherry virus 1 on apricots in hungary. / Baráth, Dániel; Jaksa-Czotter, Nikoletta; Molnár, János; Varga, Tünde; Balássy, Júlia; Szabó, Luca Krisztina; Kirilla, Zoltán; Tusnády, G.; Preininger, Éva; Várallyay, E.

In: Viruses, Vol. 10, No. 6, 318, 11.06.2018.

Research output: Article

Baráth, D, Jaksa-Czotter, N, Molnár, J, Varga, T, Balássy, J, Szabó, LK, Kirilla, Z, Tusnády, G, Preininger, É & Várallyay, E 2018, 'Small RNA NGS revealed the presence of cherry virus A and little cherry virus 1 on apricots in hungary', Viruses, vol. 10, no. 6, 318. https://doi.org/10.3390/v10060318
Baráth D, Jaksa-Czotter N, Molnár J, Varga T, Balássy J, Szabó LK et al. Small RNA NGS revealed the presence of cherry virus A and little cherry virus 1 on apricots in hungary. Viruses. 2018 jún. 11;10(6). 318. https://doi.org/10.3390/v10060318
Baráth, Dániel ; Jaksa-Czotter, Nikoletta ; Molnár, János ; Varga, Tünde ; Balássy, Júlia ; Szabó, Luca Krisztina ; Kirilla, Zoltán ; Tusnády, G. ; Preininger, Éva ; Várallyay, E. / Small RNA NGS revealed the presence of cherry virus A and little cherry virus 1 on apricots in hungary. In: Viruses. 2018 ; Vol. 10, No. 6.
@article{ff46307aa66a43febb8440855886a8d0,
title = "Small RNA NGS revealed the presence of cherry virus A and little cherry virus 1 on apricots in hungary",
abstract = "Fruit trees, such as apricot trees, are constantly exposed to the attack of viruses. As they are propagated in a vegetative way, this risk is present not only in the field, where they remain for decades, but also during their propagation. Metagenomic diagnostic methods, based on next generation sequencing (NGS), offer unique possibilities to reveal all the present pathogens in the investigated sample. Using NGS of small RNAs, a special field of these techniques, we tested leaf samples of different varieties of apricot originating from an isolator house or open field stock nursery. As a result, we identified Cherry virus A (CVA) and little cherry virus 1 (LChV-1) for the first time in Hungary. The NGS results were validated by RT-PCR and also by Northern blot in the case of CVA. Cloned and Sanger sequenced viral-specific PCR products enabled us to investigate their phylogenetic relationships. However, since these pathogens have not been described in our country before, their role in symptom development and modification during co-infection with other viruses requires further investigation.",
keywords = "Apricot, Cherry virus A, Diagnostics, Fruit tree viruses, Little cherry virus 1, Metagenomics, Small RNA NGS",
author = "D{\'a}niel Bar{\'a}th and Nikoletta Jaksa-Czotter and J{\'a}nos Moln{\'a}r and T{\"u}nde Varga and J{\'u}lia Bal{\'a}ssy and Szab{\'o}, {Luca Krisztina} and Zolt{\'a}n Kirilla and G. Tusn{\'a}dy and {\'E}va Preininger and E. V{\'a}rallyay",
year = "2018",
month = "6",
day = "11",
doi = "10.3390/v10060318",
language = "English",
volume = "10",
journal = "Viruses",
issn = "1999-4915",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "6",

}

TY - JOUR

T1 - Small RNA NGS revealed the presence of cherry virus A and little cherry virus 1 on apricots in hungary

AU - Baráth, Dániel

AU - Jaksa-Czotter, Nikoletta

AU - Molnár, János

AU - Varga, Tünde

AU - Balássy, Júlia

AU - Szabó, Luca Krisztina

AU - Kirilla, Zoltán

AU - Tusnády, G.

AU - Preininger, Éva

AU - Várallyay, E.

PY - 2018/6/11

Y1 - 2018/6/11

N2 - Fruit trees, such as apricot trees, are constantly exposed to the attack of viruses. As they are propagated in a vegetative way, this risk is present not only in the field, where they remain for decades, but also during their propagation. Metagenomic diagnostic methods, based on next generation sequencing (NGS), offer unique possibilities to reveal all the present pathogens in the investigated sample. Using NGS of small RNAs, a special field of these techniques, we tested leaf samples of different varieties of apricot originating from an isolator house or open field stock nursery. As a result, we identified Cherry virus A (CVA) and little cherry virus 1 (LChV-1) for the first time in Hungary. The NGS results were validated by RT-PCR and also by Northern blot in the case of CVA. Cloned and Sanger sequenced viral-specific PCR products enabled us to investigate their phylogenetic relationships. However, since these pathogens have not been described in our country before, their role in symptom development and modification during co-infection with other viruses requires further investigation.

AB - Fruit trees, such as apricot trees, are constantly exposed to the attack of viruses. As they are propagated in a vegetative way, this risk is present not only in the field, where they remain for decades, but also during their propagation. Metagenomic diagnostic methods, based on next generation sequencing (NGS), offer unique possibilities to reveal all the present pathogens in the investigated sample. Using NGS of small RNAs, a special field of these techniques, we tested leaf samples of different varieties of apricot originating from an isolator house or open field stock nursery. As a result, we identified Cherry virus A (CVA) and little cherry virus 1 (LChV-1) for the first time in Hungary. The NGS results were validated by RT-PCR and also by Northern blot in the case of CVA. Cloned and Sanger sequenced viral-specific PCR products enabled us to investigate their phylogenetic relationships. However, since these pathogens have not been described in our country before, their role in symptom development and modification during co-infection with other viruses requires further investigation.

KW - Apricot

KW - Cherry virus A

KW - Diagnostics

KW - Fruit tree viruses

KW - Little cherry virus 1

KW - Metagenomics

KW - Small RNA NGS

UR - http://www.scopus.com/inward/record.url?scp=85048727553&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048727553&partnerID=8YFLogxK

U2 - 10.3390/v10060318

DO - 10.3390/v10060318

M3 - Article

C2 - 29891760

AN - SCOPUS:85048727553

VL - 10

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 6

M1 - 318

ER -