A new overpressured layer chromatography (OPLC) separation procedure has been developed for single- and multi-channel separation using a non-segmented sorbent bed and flowing eluent wall (FEW) for operating segmentation. The FEW detaches the sorbent bed into active and nonactive parts regarding separation during the process. Only mobile phase is introduced into the non-active part, while, for the active part, eluent and also the sample can be admitted, thus the non-homogeneous part of the sorbent bed is excluded from the separation process. The FEW helps the elimination of the edge effect of OPLC in case of single sample injection and abolition of the sample mixing effect of neighboring lanes in the case of a multi-channel separation process. In the case of dirty samples, the one channel FEW-OPLC system is well suited for quick isolation in different preparative ranges using preparative chromatoplates. The multichannel solution will be a tool for high throughput analysis using efficient fine, superfine, or monolithic layers. The four-channel version can be applied for high throughput multi (parallel) analysis, as well as micro- and semi-preparative parallel isolation using efficient analytical or preparative layers. The FEW provides the possibility for real multi-channel liquid chromatographic separation on a non-segmented sorbent bed.
|Number of pages||17|
|Journal||Journal of Liquid Chromatography and Related Technologies|
|Publication status||Published - okt. 13 2003|
ASJC Scopus subject areas
- Analytical Chemistry
- Pharmaceutical Science
- Clinical Biochemistry