Abstract
To simultaneously detect Sudan dyes and carotenoids in red pepper and tomato products, high-performance liquid chromatography (HPLC) methods with photodiode-array detection are developed and validated. The methods include the use of end-capped and nonend-capped adsorbents with a gradient elution system starting with water containing methanol. Water content of 9% in the starting mobile phase is found to be necessary to ensure sufficient separation of Sudan dyes and to avoid overlapping or interference with the carotenoids of considerable content. The data of the validation reveal the accuracy and precision of the developed methods. A limit of Sudan dyes detection of 1-5 μg/g in red pepper or tomato sauce could be approached. The methods provide excellent separation of the carotenoids from the unsaponified extracts of red pepper and the tomato products.
| Original language | English |
|---|---|
| Pages (from-to) | 461-465 |
| Number of pages | 5 |
| Journal | Journal of Chromatographic Science |
| Volume | 43 |
| Issue number | 9 |
| Publication status | Published - okt. 2005 |
Fingerprint
ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry
Cite this
Simultaneous determination of Sudan dyes and carotenoids in red pepper and tomato products by HPLC. / Daood, H.; Biacs, P.
In: Journal of Chromatographic Science, Vol. 43, No. 9, 10.2005, p. 461-465.Research output: Article
}
TY - JOUR
T1 - Simultaneous determination of Sudan dyes and carotenoids in red pepper and tomato products by HPLC
AU - Daood, H.
AU - Biacs, P.
PY - 2005/10
Y1 - 2005/10
N2 - To simultaneously detect Sudan dyes and carotenoids in red pepper and tomato products, high-performance liquid chromatography (HPLC) methods with photodiode-array detection are developed and validated. The methods include the use of end-capped and nonend-capped adsorbents with a gradient elution system starting with water containing methanol. Water content of 9% in the starting mobile phase is found to be necessary to ensure sufficient separation of Sudan dyes and to avoid overlapping or interference with the carotenoids of considerable content. The data of the validation reveal the accuracy and precision of the developed methods. A limit of Sudan dyes detection of 1-5 μg/g in red pepper or tomato sauce could be approached. The methods provide excellent separation of the carotenoids from the unsaponified extracts of red pepper and the tomato products.
AB - To simultaneously detect Sudan dyes and carotenoids in red pepper and tomato products, high-performance liquid chromatography (HPLC) methods with photodiode-array detection are developed and validated. The methods include the use of end-capped and nonend-capped adsorbents with a gradient elution system starting with water containing methanol. Water content of 9% in the starting mobile phase is found to be necessary to ensure sufficient separation of Sudan dyes and to avoid overlapping or interference with the carotenoids of considerable content. The data of the validation reveal the accuracy and precision of the developed methods. A limit of Sudan dyes detection of 1-5 μg/g in red pepper or tomato sauce could be approached. The methods provide excellent separation of the carotenoids from the unsaponified extracts of red pepper and the tomato products.
UR - http://www.scopus.com/inward/record.url?scp=27744600999&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27744600999&partnerID=8YFLogxK
M3 - Article
C2 - 16212791
AN - SCOPUS:27744600999
VL - 43
SP - 461
EP - 465
JO - Journal of Chromatographic Science
JF - Journal of Chromatographic Science
SN - 0021-9665
IS - 9
ER -