Sex-dependent and differential responses to acute restraint stress of corticotropin-releasing factor-producing neurons in the rat paraventricular nucleus, central amygdala, and bed nucleus of the stria terminalis

Linda Sterrenburg, B. Gaszner, Jeroen Boerrigter, Lennart Santbergen, Mattia Bramini, Eric W. Roubos, Bernard W M M Peeters, Tamás Kozicz

Research output: Article

56 Citations (Scopus)

Abstract

Male and female rodents respond differently to acute stress. We tested our hypothesis that this sex difference is based on differences in stress sensitivity of forebrain areas, by determining possible effects of a single acute psychogenic stressor (1-hr restraint stress) on neuronal gene expression (c-Fos and FosB immunoreactivities), storage of corticotropin-releasing factor (CRF) immunoreactivity, and CRF production (CRF mRNA in situ hybridization) as well as the expression of genes associated with epigenetic processes (quantitative RT-PCR) in the rat paraventricular nucleus (PVN), the oval and fusiform subdivisions of the bed nucleus of the stria terminalis (BSTov and BSTfu, respectively), and the central amygdala (CeA), in both males and females. Compared with females, male rats responded to the stressor with a stronger rise in corticosterone titer and a stronger increase in neuronal contents of c-Fos, CRF mRNA, and CREB-binding protein mRNA in the PVN. In the BSTov, females but not males showed an increase in c-Fos, whereas the CRF mRNA content was increased in males only. In the BSTfu, males and females showed similar stress-induced increases in c-Fos and FosB, whereas in the CeA, both sexes revealed similar increases in c-Fos and in CRF mRNA. We conclude that male and female rats differ in their reactivity to acute stress with respect to possibly epigenetically mediated (particularly in the PVN) neuronal gene expression and neuropeptide dynamics (PVN and BSTov) and that this difference may contribute to the sex dependence of the animal's physiological and behavioral responses to an acute stressor.

Original languageEnglish
Pages (from-to)179-192
Number of pages14
JournalJournal of Neuroscience Research
Volume90
Issue number1
DOIs
Publication statusPublished - jan. 2012

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Septal Nuclei
Paraventricular Hypothalamic Nucleus
Corticotropin-Releasing Hormone
Amygdala
Neurons
Messenger RNA
Gene Expression
Genetic Epigenesis
CREB-Binding Protein
Corticosterone
Prosencephalon
Central Amygdaloid Nucleus
Neuropeptides
Sex Characteristics
In Situ Hybridization
Rodentia
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Sex-dependent and differential responses to acute restraint stress of corticotropin-releasing factor-producing neurons in the rat paraventricular nucleus, central amygdala, and bed nucleus of the stria terminalis. / Sterrenburg, Linda; Gaszner, B.; Boerrigter, Jeroen; Santbergen, Lennart; Bramini, Mattia; Roubos, Eric W.; Peeters, Bernard W M M; Kozicz, Tamás.

In: Journal of Neuroscience Research, Vol. 90, No. 1, 01.2012, p. 179-192.

Research output: Article

Sterrenburg, Linda ; Gaszner, B. ; Boerrigter, Jeroen ; Santbergen, Lennart ; Bramini, Mattia ; Roubos, Eric W. ; Peeters, Bernard W M M ; Kozicz, Tamás. / Sex-dependent and differential responses to acute restraint stress of corticotropin-releasing factor-producing neurons in the rat paraventricular nucleus, central amygdala, and bed nucleus of the stria terminalis. In: Journal of Neuroscience Research. 2012 ; Vol. 90, No. 1. pp. 179-192.
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abstract = "Male and female rodents respond differently to acute stress. We tested our hypothesis that this sex difference is based on differences in stress sensitivity of forebrain areas, by determining possible effects of a single acute psychogenic stressor (1-hr restraint stress) on neuronal gene expression (c-Fos and FosB immunoreactivities), storage of corticotropin-releasing factor (CRF) immunoreactivity, and CRF production (CRF mRNA in situ hybridization) as well as the expression of genes associated with epigenetic processes (quantitative RT-PCR) in the rat paraventricular nucleus (PVN), the oval and fusiform subdivisions of the bed nucleus of the stria terminalis (BSTov and BSTfu, respectively), and the central amygdala (CeA), in both males and females. Compared with females, male rats responded to the stressor with a stronger rise in corticosterone titer and a stronger increase in neuronal contents of c-Fos, CRF mRNA, and CREB-binding protein mRNA in the PVN. In the BSTov, females but not males showed an increase in c-Fos, whereas the CRF mRNA content was increased in males only. In the BSTfu, males and females showed similar stress-induced increases in c-Fos and FosB, whereas in the CeA, both sexes revealed similar increases in c-Fos and in CRF mRNA. We conclude that male and female rats differ in their reactivity to acute stress with respect to possibly epigenetically mediated (particularly in the PVN) neuronal gene expression and neuropeptide dynamics (PVN and BSTov) and that this difference may contribute to the sex dependence of the animal's physiological and behavioral responses to an acute stressor.",
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AU - Boerrigter, Jeroen

AU - Santbergen, Lennart

AU - Bramini, Mattia

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